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PMID: 2681144 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus).

Journal of bacteriology ·Vol. 171 ·No. 11 ·1989-11-00 ·Pages 5872-81

Vara J, Lewandowska-Skarbek M, Wang YG, Donadio S, Hutchinson CR

Abstract

Genes that govern the formation of deoxysugars or their attachment to erythronolide B and 3 alpha-mycarosyl erythronolide B, intermediates of the biosynthesis of the 14-membered macrolide antibiotic erythromycin, were cloned from Saccharopolyspora erythraea (formerly Streptomyces erythreus). Segments of DNA that complement the eryB25, eryB26, eryB46, eryC1-60, and eryD24 mutations blocking the formation of erythronolide B or 3 alpha-mycarosyl erythronolide B, when cloned in Escherichia coli-Streptomyces shuttle cosmids or plasmid vectors that can transform S. erythraea, were located in a ca. 18-kilobase-pair region upstream of the erythromycin resistance (ermE) gene. The eryC1 gene lies just to the 5' side of ermE, and one (or possibly two) eryB gene is approximately 12 kilobase pairs farther upstream. Another eryB gene may be in the same region, while an additional eryB mutation appears to be located elsewhere. The eryD gene lies between the eryB and eryC1 genes and may regulate their function on the basis of the phenotype of an EryD- mutant.

MeSH Terms
Cloning, Molecular DNA, Bacterial/genetics Deoxy Sugars/biosynthesis Drug Resistance, Microbial/genetics Erythromycin/biosynthesis Escherichia coli/genetics Genes, Bacterial Mutation Nucleic Acid Hybridization Plasmids R Factors Restriction Mapping Streptomyces/genetics,metabolism Transformation, Bacterial
Chemicals
DNA, Bacterial Deoxy Sugars Erythromycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vara J
School of Pharmacy, University of Wisconsin, Madison 53706.
Lewandowska-Skarbek M
Wang Y G
Donadio S
Hutchinson C R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.11.5872-5881.1989
Published
1989-11-00
Pages
5872-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210448
Subset
IM
Grants
NIGMS NIH HHS · GM31925 · United States
External Links
PubMed source DOI: 10.1128/jb.171.11.5872-5881.1989 PMC Full Text View journal details
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