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PMID: 2682641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transformation stimulates glucose transporter gene expression in the absence of protein kinase C.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 21 ·1989-11-00 ·Pages 8252-6

Hiraki Y, Garcia de Herreros A, Birnbaum MJ

Abstract

The rat brain glucose transporter (GT) gene is rapidly activated coincident with the initiation of growth in response to oncogenic transformation or the addition of growth factors to quiescent fibroblasts. The latter response has been shown to be mediated by protein kinase C-dependent and-independent pathways. We studied the role of protein kinase C in the transformation-induced activation of the GT gene. Transformation of fibroblasts by either the v-fps or the Ki-ras oncogene rapidly increased the levels of GT mRNA. Either viral oncogene remained capable of stimulating the GT gene after depletion of cellular protein kinase C by prolonged pretreatment of fibroblasts with phorbol 12-myristate 13-acetate. These data indicate that protein kinase C is not required for the rapid activation of gene transcription by oncogenic transformation.

MeSH Terms
Animals Brain/metabolism Cell Line Cell Transformation, Neoplastic Gene Expression Regulation Genes, ras Humans Kinetics Monosaccharide Transport Proteins/genetics Oncogenes Protein Kinase C/metabolism RNA, Messenger/drug effects,genetics Rats Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic
Chemicals
Monosaccharide Transport Proteins RNA, Messenger Protein Kinase C Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hiraki Y
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02115.
Garcia de Herreros A
Birnbaum M J
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.21.8252
Published
1989-11-00
Pages
8252-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298258
Subset
IM
Grants
NIDDK NIH HHS · DK39519 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.21.8252 PMC Full Text View journal details
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