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PMID: 2686986 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

tRNA hopping: enhancement by an expanded anticodon.

The EMBO journal ·Vol. 8 ·No. 13 ·1989-12-20 ·Pages 4315-23

O'Connor M, Gesteland RF, Atkins JF

Abstract

At a low level wild-type tRNA(1Val) inserts a single amino acid (valine) for the five nucleotide sequence GUGUA which has overlapping valine codons. Mutants of tRNA(1Val) with an insertion of A or U between positions 34 and 35 of their anticodons have enhanced reading of the quintuplet sequences. We propose that this decoding occurs by a hopping mechanism rather than by quintuplet pairing. Such hopping involves disengagement of the paired codon and anticodon with the mRNA slipping two (or more) bases along the ribosomal--peptidyl tRNA complex and subsequently re-pairing at a second codon--the landing site. The mutant with the anticodon sequence 3'CAAU5' 'hops' over the stop codon in the mRNA sequence GUG UAA GUU with the insertion of a single amino acid (valine). In contrast, in reading the same sequence, the mutant with the anticodon 3'CAUU5' hops onto the stop with the insertion of two valine residues. It is likely that in some instances of hopping alternate anticodon bases are used for the initial pairing and at the landing site.

MeSH Terms

Alleles Amino Acid Sequence Anticodon/genetics Base Composition Base Sequence Cloning, Molecular Codon/genetics Cosmids Escherichia coli/genetics Molecular Sequence Data Mutation Nucleic Acid Conformation RNA, Transfer/genetics RNA, Transfer, Amino Acid-Specific/genetics RNA, Transfer, Val/genetics

Chemicals

Anticodon Codon RNA, Transfer, Amino Acid-Specific RNA, Transfer, Val RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

O'Connor M

Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132.

Gesteland R F

Atkins J F

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24 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1989-12-20

Pages

4315-23

Language

English

Region

England

NLM ID

8208664

PMCID

PMC401642

Subset

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PubMed source PMC Full Text View journal details

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tRNA hopping: enhancement by an expanded anticodon.

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