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PMID: 2846182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region.

Cell ·Vol. 55 ·No. 3 ·1988-11-04 ·Pages 447-58

Jacks T, Madhani HD, Masiarz FR, Varmus HE

Abstract

The gag-pol protein of Rous sarcoma virus (RSV), the precursor to the enzymes responsible for reverse transcription and integration, is expressed from two genes that lie in different translational reading frames by ribosomal frameshifting. Here, we localize the site of frameshifting and show that the frameshifting reaction is mediated by slippage of two adjacent tRNAs by a single nucleotide in the 5' direction. The gag terminator, which immediately follows the frameshift site, is not required for frameshifting. Other suspected retroviral frameshift sites mediate frameshifting when placed at the end of RSV gag. Mutations in RSV pol also affect synthesis of the gag-pol protein in vitro. The effects of these mutations best correlate with the potential to form an RNA stem-loop structure adjacent to the frameshift site. A short sequence of RSV RNA, 147 nucleotides in length, containing the frameshift site and stem-loop structure, is sufficient to direct frameshifting in a novel genetic context.

MeSH Terms
Amino Acid Sequence Avian Sarcoma Viruses/genetics Base Sequence Chromosome Deletion Gene Products, gag Genes, Viral Molecular Sequence Data Mutation Protein Biosynthesis Protein Conformation RNA, Transfer, Leu/genetics RNA, Viral/genetics Retroviridae Proteins/genetics Ribosomes/physiology
Chemicals
Gene Products, gag RNA, Transfer, Leu RNA, Viral Retroviridae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jacks T
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Madhani H D
Masiarz F R
Varmus H E
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(88)90031-1
Published
1988-11-04
Pages
447-58
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133365
Subset
IM
External Links
PubMed source DOI: 10.1016/0092-8674(88)90031-1 PMC Full Text View journal details
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