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PMID: 2703821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Influence of temperature on the calcium sensitivity of the myofilaments of skinned ventricular muscle from the rabbit.

The Journal of general physiology ·Vol. 93 ·No. 3 ·1989-03-00 ·Pages 411-28

Harrison SM, Bers DM

Abstract

The steady-state myofilament Ca sensitivity was determined in skinned cardiac trabeculae from the rabbit right ventricle (diameter, 0.13-0.34 mm) at 36, 29, 22, 15, 8, and 1 degree C. Muscles were stimulated to 0.5 Hz and stretched to a length at which maximum twitch tension was generated. The preparation was then skinned with 1% vol/vol Triton X-100 in a relaxing medium (10 mM EGTA, pCa 9.0). Each preparation was exposed to a series of Ca-containing solutions (pCa 6.3-4.0) at two of the six temperatures studied (temperature was regulated to +/- 0.1 degree C). The pCa values (mean +/- SD, n = 6) corresponding to half maximal tension at 36, 29, 22, 15, 8, and 1 degree C were 5.47 +/- 0.07, 5.49 +/- 0.07, 5.34 +/- 0.05, 5.26 +/- 0.09, 4.93 +/- 0.06, and 4.73 +/- 0.04, respectively. Mean (+/- SD) maximum tension (Cmax) developed by the preparation as a percentage of that at 22 degrees C was 118 +/- 10, 108 +/- 5, 74 +/- 6, 57 +/- 7, and 29 +/- 5% at 36, 29, 15, 8, and 1 degree C, respectively. As cooling led to a shift of Ca sensitivity towards higher [Ca2+] and a reduction of Cmax, the Ca sensitivity curves over this range of temperatures do not cross over as has been described for canine Purkinje fibers (Fabiato 1985). Since tension is decreased by cooling at all levels of [Ca2+] it is unlikely that changes in myofilament Ca sensitivity play a role in the large hypothermic inotropy seen in rabbit ventricular muscle. The increase in sensitivity of the myofilaments to Ca on warming from 1 to 29 degrees C might be related to the increase in force seen on rewarming from a rapid cooling contracture in intact rabbit ventricular muscle.

MeSH Terms
Actin Cytoskeleton/drug effects Animals Calcium/pharmacology Cytoskeleton/drug effects Electric Stimulation In Vitro Techniques Male Myocardial Contraction/drug effects Rabbits Temperature
Chemicals
Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harrison S M
Division of Biomedical Sciences, University of California, Riverside 92521.
Bers D M
References (52)
52 references, click to expand
  1. Thermodynamic quantities associated with the interaction of adenosine triphosphate with metal ions.
    J Am Chem Soc. 1966 Feb 20;88(4):668-71 PMID: 5902558
  2. Reconsideration of the ultrastructural basis of cardiac length-tension relations.
    Circ Res. 1974 Oct;35(4):517-26 PMID: 4606992
  3. Hydrogen ion buffers for biological research.
    Biochemistry. 1966 Feb;5(2):467-77 PMID: 5942950
  4. Carnosine and related substances in animal tissues.
    Comp Biochem Physiol. 1970 May 1;34(1):3-30 PMID: 4988625
  5. Influence of temperature upon contractile activation and isometric force production in mechanically skinned muscle fibers of the frog.
    J Gen Physiol. 1982 Aug;80(2):279-97 PMID: 6981684
  6. Temperature-dependence of shortening velocity and rate of isometric tension development in rat skeletal muscle.
    J Physiol. 1982 Aug;329:465-83 PMID: 7143257
  7. Calcium- and length-dependent force production in rat ventricular muscle.
    J Physiol. 1982 Aug;329:527-40 PMID: 7143258
  8. EGTA purity and the buffering of calcium ions in physiological solutions.
    Am J Physiol. 1984 Jan;246(1 Pt 1):C160-6 PMID: 6421170
  9. Kinetic properties and the functional role of particulate MM-isoenzyme of creatine phosphokinase bound to heart muscle myofibrils.
    FEBS Lett. 1976 Mar 1;62(3):293-6 PMID: 1278371
  10. Kinetics of reaction in calcium-activated skinned muscle fibres.
    Nature. 1976 Aug 12;262(5569):610-3 PMID: 958428
  11. Direct measurement of the intracellular pH of mammalian cardiac muscle.
    J Physiol. 1976 Nov;262(3):755-71 PMID: 13205
  12. Micro-electrode measurement of the intracellular pH and buffering power of mouse soleus muscle fibres.
    J Physiol. 1977 Jun;267(3):791-810 PMID: 17740
  13. Tension responses to sudden length change in stimulated frog muscle fibres near slack length.
    J Physiol. 1977 Jul;269(2):441-515 PMID: 302333
  14. Effect of changing the composition of the bathing solutions upon the isometric tension-pCa relationship in bundles of crustacean myofibrils.
    J Physiol. 1977 Sep;270(3):627-52 PMID: 20499
  15. Regulation of tension in skinned muscle fibers: effect of high concentrations of Mg-ATP.
    Am J Physiol. 1977 Nov;233(5):C127-34 PMID: 411376
  16. Calcium and strontium concentration changes within skinned muscle preparations following a change in the external bathing solution.
    J Physiol. 1978 Feb;275:241-62 PMID: 24736
  17. Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiace and skeletal muscles.
    J Physiol. 1978 Mar;276:233-55 PMID: 25957
  18. Calcium-induced release of calcium from the sarcoplasmic reticulum of skinned cells from adult human, dog, cat, rabbit, rat, and frog hearts and from fetal and new-born rat ventricles.
    Ann N Y Acad Sci. 1978 Apr 28;307:491-522 PMID: 360947
  19. Investigation of the temperature dependence of the cross bridge parameters for attachment, force generation and detachment as deduced from mechano-chemical studies in glycerinated single fibres from the dorsal longitudinal muscle of Lethocerus maximus.
    Biophys Struct Mech. 1979 Dec;6(1):1-29 PMID: 540123
  20. Re-examination of the apparent binding constant of ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid with calcium around neutral pH.
    J Biochem. 1980 May;87(5):1305-12 PMID: 6771253
  21. Effects of calcium on the sarcomere length-tension relation in rat cardiac muscle. Implications for the Frank-Starling mechanism.
    Circ Res. 1980 Oct;47(4):610-19 PMID: 7408137
  22. Characterization of the electrogenic sodium pump in cardiac Purkinje fibres.
    J Physiol. 1980 Jun;303:441-74 PMID: 7431244
  23. Cardiac contractility and sarcolemmal calcium binding in several cardiac muscle preparations.
    Am J Physiol. 1981 Apr;240(4):H576-83 PMID: 6971579
  24. A common source of error in pH measurements.
    Biochem J. 1981 Apr 1;195(1):259-62 PMID: 7306051
  25. Myoplasmic free calcium concentration reached during the twitch of an intact isolated cardiac cell and during calcium-induced release of calcium from the sarcoplasmic reticulum of a skinned cardiac cell from the adult rat or rabbit ventricle.
    J Gen Physiol. 1981 Nov;78(5):457-97 PMID: 6796647
  26. Calcium-activated force responses in fast- and slow-twitch skinned muscle fibres of the rat at different temperatures.
    J Physiol. 1981 Aug;317:281-302 PMID: 7310735
  27. A simple method for the accurate determination of free [Ca] in Ca-EGTA solutions.
    Am J Physiol. 1982 May;242(5):C404-8 PMID: 6805332
  28. The inhibitory effects of monovalent ions on force development in detergent-skinned ventricular muscle from guinea-pig.
    J Physiol. 1984 Jul;352:353-74 PMID: 6747893
  29. Differences in temperature dependence of muscle contractile properties and myofibrillar ATPase activity in a cold-temperature fish.
    J Exp Biol. 1984 Jul;111:179-89 PMID: 6238119
  30. The hill coefficient for the Ca2+-activation of striated muscle contraction.
    Biophys J. 1984 Oct;46(4):541-3 PMID: 6498270
  31. Time and calcium dependence of activation and inactivation of calcium-induced release of calcium from the sarcoplasmic reticulum of a skinned canine cardiac Purkinje cell.
    J Gen Physiol. 1985 Feb;85(2):247-89 PMID: 2580043
  32. Potentiometric measurements of stoichiometric and apparent affinity constants of EGTA for protons and divalent ions including calcium.
    Biochim Biophys Acta. 1985 May 8;839(3):287-99 PMID: 2985122
  33. Temperature-dependent calcium sensitivity changes in skinned muscle fibres of rat and toad.
    J Physiol. 1985 Mar;360:1-12 PMID: 3921690
  34. Effects of rapid cooling on mechanical and electrical responses in ventricular muscle of guinea-pig.
    J Physiol. 1985 Apr;361:361-78 PMID: 3989731
  35. The contractile behaviour of EGTA- and detergent-treated heart muscle.
    J Muscle Res Cell Motil. 1985 Oct;6(5):541-67 PMID: 3934211
  36. Temperature-induced transitory and steady-state changes in the calcium current of guinea pig ventricular myocytes.
    Pflugers Arch. 1985 Oct;405(3):294-6 PMID: 2415920
  37. Relationship between force and intracellular [Ca2+] in tetanized mammalian heart muscle.
    J Gen Physiol. 1986 Feb;87(2):223-42 PMID: 2419483
  38. Tension-pCa relations of saponin-skinned rabbit and human heart muscle.
    J Mol Cell Cardiol. 1986 Jan;18(1):55-66 PMID: 3950971
  39. The effects of inorganic phosphate and creatine phosphate on force production in skinned muscles from rat ventricle.
    J Physiol. 1986 Jan;370:585-604 PMID: 3958986
  40. The determination of binding constants with a differential thermal and potentiometric titration apparatus. II. EDTA, EGTA and calcium.
    J Biochem Biophys Methods. 1986 Mar;12(3):135-46 PMID: 3086421
  41. Comparison between the sarcomere length-force relations of intact and skinned trabeculae from rat right ventricle. Influence of calcium concentrations on these relations.
    Circ Res. 1986 Jun;58(6):755-68 PMID: 3719928
  42. Sodium/calcium exchange and intracellular calcium buffering in ferret myocardium: an ion-sensitive micro-electrode study.
    J Physiol. 1986 Apr;373:163-79 PMID: 2427694
  43. Relationships between the sarcoplasmic reticulum and sarcolemmal calcium transport revealed by rapidly cooling rabbit ventricular muscle.
    J Gen Physiol. 1986 Oct;88(4):437-73 PMID: 3783123
  44. The mechanism of ryanodine action in rabbit ventricular muscle evaluated with Ca-selective microelectrodes and rapid cooling contractures.
    Can J Physiol Pharmacol. 1987 Apr;65(4):610-8 PMID: 3607606
  45. The effect of temperature and ionic strength on the apparent Ca-affinity of EGTA and the analogous Ca-chelators BAPTA and dibromo-BAPTA.
    Biochim Biophys Acta. 1987 Aug 13;925(2):133-43 PMID: 3113491
  46. Ryanodine and the calcium content of cardiac SR assessed by caffeine and rapid cooling contractures.
    Am J Physiol. 1987 Sep;253(3 Pt 1):C408-15 PMID: 3631249
  47. Inotropic response to hypothermia and the temperature-dependence of ryanodine action in isolated rabbit and rat ventricular muscle: implications for excitation-contraction coupling.
    Circ Res. 1987 Dec;61(6):761-71 PMID: 3677335
  48. Mechanisms contributing to the cardiac inotropic effect of Na pump inhibition and reduction of extracellular Na.
    J Gen Physiol. 1987 Oct;90(4):479-504 PMID: 3681259
  49. Hysteresis and the length dependence of calcium sensitivity in chemically skinned rat cardiac muscle.
    J Physiol. 1988 Jul;401:115-43 PMID: 3171985
  50. SR Ca loading in cardiac muscle preparations based on rapid-cooling contractures.
    Am J Physiol. 1989 Jan;256(1 Pt 1):C109-20 PMID: 2536224
  51. Intracellular pH and buffer curves of cardiac muscle in rats as affected by temperature.
    Respir Physiol. 1973 Jul;18(2):161-70 PMID: 4730748
  52. Thermodynamic studies of the formation and ionization of the magnesium(II) complexes of ADP and ATP over the pH range 5 to 9.
    J Am Chem Soc. 1966 Jun 20;88(12):2631-40 PMID: 5941264
Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.93.3.411
Published
1989-03-00
Pages
411-28
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2216215
Subset
IM
Grants
NHLBI NIH HHS · HL-01526 · United States
NHLBI NIH HHS · HL-30077 · United States
External Links
PubMed source DOI: 10.1085/jgp.93.3.411 PMC Full Text View journal details
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