Home LiteratureArticle Details
PMID: 270708 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Immunological distinction between acetylcholine receptor and the alpha-bungarotoxin-binding component on sympathetic neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 10 ·1977-10-00 ·Pages 4689-92

Patrick J, Stallcup WB

Abstract

Clone PC12 of a rat sympathetic neuron cell line binds alpha-bungarotoxin and exhibits carbamoylcholine-stimulated uptake of sodium ions. Concentrations of alpha-bungarotoxin that saturate the alpha-bungarotoxin-binding site have no effect on agonist-stimulated sodium uptake. Conversely, antibodies against eel acetylcholine receptor block the agonist-induced sodium flux but fail to recognize the alpha-bungarotoxin-binding component. Detergent extracts of the PC12 clone inhibit the ability of antibody to eel acetylcholine receptor to recognize 125I-alpha-bungarotoxin-acetylcholine receptor complexes derived from muscle. These results distinguish between a ganglionic nicotinic acetylcholine receptor and an alpha-bungarotoxin-binding component on these cells, and provide evidence for antigenic similarities between muscle acetylcholine receptor and ganglionic acetylcholine receptor.

MeSH Terms
Acetylcholine/metabolism Binding Sites Bungarotoxins/metabolism Cell Line Neurons/metabolism Receptors, Cholinergic/immunology Sodium/metabolism Sympathetic Nervous System/metabolism
Chemicals
Bungarotoxins Receptors, Cholinergic Sodium Acetylcholine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Patrick J
Stallcup W B
References (21)
21 references, click to expand
  1. Cholinergic metabolism and synapse formation by a rat nerve cell line.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2579-83 PMID: 196294
  2. Modulation of acetylcholine receptor by antibody against the receptor.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):3090-4 PMID: 268657
  3. Acetylcholine receptor metabolism in a nonfusing muscle cell line.
    J Biol Chem. 1977 Mar 25;252(6):2143-53 PMID: 845167
  4. -Bungarotoxin used as a probe for acetylcholine receptors of cultured neurones.
    Nature. 1973 May 18;243(5403):163-6 PMID: 4550035
  5. Studies on purified eel acetylcholine receptor and anti-acetylcholine receptor antibody.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3334-8 PMID: 4128544
  6. Clonal cell lines from the rat central nervous system.
    Nature. 1974 May 17;249(454):224-7 PMID: 4151463
  7. Effect of whole and fractionated cobra venom on sympathetic ganglionic transmission.
    Eur J Pharmacol. 1969 Dec;8(3):326-30 PMID: 4391828
  8. Immunological characterisation of the cholinergic receptor protein from Electrophorus electricus.
    FEBS Lett. 1973 Sep 1;35(1):124-8 PMID: 4201666
  9. Development of acetylcholine receptor clusters on cultured muscle cells.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):270-4 PMID: 4509661
  10. Acetylcholine receptors of muscle grown in vitro.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3180-4 PMID: 4508312
  11. Appearance of acetylcholine receptors during differentiation of a myogenic cell line.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2762-6 PMID: 4507600
  12. Nicotinic cholinergic receptor in brain detected by binding of alpha-(3 H) bungarotoxin.
    Biochim Biophys Acta. 1974 Sep 5;362(2):346-55 PMID: 4424687
  13. Binding of (125I)-alpha bungarotoxin to particulate fractions of rat and guinea pig brain.
    Biochem Biophys Res Commun. 1973 Dec 19;55(4):1311-8 PMID: 4771998
  14. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8 PMID: 1065897
  15. Binding of alpha-bungarotoxin to chick sympathetic ganglia: properties of the receptor and its rate of appearance during developement.
    Brain Res. 1976 Jul 23;111(1):135-45 PMID: 953693
  16. Formation of cholinergic synapses between dissociated sympathetic neurons and skeletal myotubes of the rat in cell culture.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1955-9 PMID: 1057775
  17. Alpha-bungarotoxin, cobra neurotoxin and excitation of Renshaw cells by acetylcholine.
    Brain Res. 1976 Apr 30;107(1):166-70 PMID: 1268719
  18. Solubilization of an alpha-bungarotoxin-binding component from rat brain.
    Biochemistry. 1976 Apr 6;15(7):1522-7 PMID: 1259953
  19. Electrical properties of a clonal cell line as determined by measurement of ion fluxes.
    Exp Cell Res. 1976 Mar 15;98(2):277-84 PMID: 943300
  20. Sodium transport by the acetylcholine receptor of cultured muscle cells.
    J Biol Chem. 1975 Mar 10;250(5):1776-81 PMID: 1167546
  21. Modes of actions of purified toxins from elapid venoms on neuromuscular transmission.
    Mem Inst Butantan. 1966;33(2):555-72 PMID: 6002427
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.10.4689
Published
1977-10-00
Pages
4689-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432013
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.10.4689 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2