Home LiteratureArticle Details
PMID: 2710113 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The C-terminal domain of transcription factor IIIA interacts differently with different 5S RNA genes.

Molecular and cellular biology ·Vol. 9 ·No. 2 ·1989-02-00 ·Pages 499-514

Xing YY, Worcel A

Abstract

DNase I footprints and affinity measurements showed that the C-terminal arm of Xenopus transcription factor IIIA interacts differently with different Xenopus 5S DNAs, forming three distinct types of transcription factor IIIA-5S DNA complexes: a somatic type, a major-oocyte (and pseudogene) type, and a trace-oocyte type. Site-directed mutagenesis on the major-oocyte 5S gene revealed that somatic-type changes at positions 53, 55, and 56 changed the structure of the transcription factor IIIA-5S DNA complex from major-oocyte to somatic, and a single trace-oocyte change at position 56 caused the change from major-oocyte to trace-oocyte complex. We further show that the somatic-type changes are accompanied by a marked enhancement in the rate of 5S RNA transcription, and we discuss the possible biological relevance of these findings.

MeSH Terms
Animals Base Sequence Binding Sites Deoxyribonuclease I Female Molecular Sequence Data Mutation Oocytes/metabolism Plasmids RNA, Ribosomal/genetics RNA, Ribosomal, 5S/genetics,isolation & purification,metabolism Transcription Factor TFIIIA Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic Xenopus laevis
Chemicals
RNA, Ribosomal RNA, Ribosomal, 5S Transcription Factor TFIIIA Transcription Factors Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Xing Y Y
Department of Biology, University of Rochester, New York 14627.
Worcel A
References (52)
52 references, click to expand
  1. The role of stable complexes that repress and activate eucaryotic genes.
    Cell. 1984 Jun;37(2):359-65 PMID: 6722879
  2. The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
    Cell. 1984 Jul;37(3):903-13 PMID: 6540147
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Chromatin assembly in Xenopus oocytes: in vitro studies.
    Cell. 1984 May;37(1):33-41 PMID: 6327057
  5. Deletion of the N-terminal region of Xenopus transcription factor IIIA inhibits specific binding to the 5 S RNA gene.
    J Biol Chem. 1988 Feb 5;263(4):1607-10 PMID: 2448298
  6. Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
    Cell. 1981 Apr;24(1):261-70 PMID: 6263489
  7. Transcription of Xenopus 5S ribosomal RNA genes.
    Nature. 1982 Jan 14;295(5845):101-5 PMID: 7057877
  8. Chromosomal mapping of Xenopus 5S genes: somatic-type versus oocyte-type.
    Nucleic Acids Res. 1983 Apr 25;11(8):2313-23 PMID: 6687939
  9. Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro.
    EMBO J. 1988 Apr;7(4):1071-9 PMID: 3402432
  10. Mapping functional regions of transcription factor TFIIIA.
    Mol Cell Biol. 1988 Apr;8(4):1684-96 PMID: 2837652
  11. Early replication and expression of oocyte-type 5S RNA genes in a Xenopus somatic cell line carrying a translocation.
    Proc Natl Acad Sci U S A. 1986 Jul;83(14):5150-4 PMID: 3460086
  12. Restoration of binding of oxidized transcription factor IIIA to 5S RNA by thioredoxin.
    Nucleic Acids Res. 1988 Sep 12;16(17):8497-507 PMID: 3419924
  13. A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4170-4 PMID: 7001457
  14. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer.
    Cell. 1978 Apr;13(4):701-16 PMID: 566163
  15. Kinetics and mechanism in the reaction of gene regulatory proteins with DNA.
    J Mol Biol. 1984 Jan 25;172(3):263-82 PMID: 6319716
  16. Temporal order of replication of Xenopus laevis 5S ribosomal RNA genes in somatic cells.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2924-8 PMID: 3458252
  17. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels.
    Anal Biochem. 1979 Sep 15;98(1):231-7 PMID: 94518
  18. Differential 5S RNA gene expression in vitro.
    Cell. 1987 Dec 4;51(5):733-40 PMID: 3677171
  19. The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
    Cell. 1981 Mar;23(3):665-9 PMID: 7226225
  20. Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
    J Biol Chem. 1980 Dec 25;255(24):11986-91 PMID: 7440579
  21. Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
    Cell. 1982 Feb;28(2):413-21 PMID: 7060135
  22. Location of the genes for 5S ribosomal RNA in Xenopus laevis.
    Chromosoma. 1973;42(2):191-203 PMID: 4726606
  23. Transcription by RNA polymerase III.
    Annu Rev Biochem. 1988;57:873-914 PMID: 3052292
  24. Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
    Cell. 1984 Sep;38(2):511-21 PMID: 6540626
  25. Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
    Cell. 1980 May;20(1):131-41 PMID: 6248230
  26. The isolation and characterization of a second oocyte 5s DNA from Xenopus laevis.
    Cell. 1977 Dec;12(4):1045-56 PMID: 563770
  27. Mapping of the sites of protection on a 5 S RNA gene by the Xenopus transcription factor IIIA. A model for the interaction.
    J Mol Biol. 1986 Dec 5;192(3):577-91 PMID: 3560227
  28. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
    Cell. 1980 Mar;19(3):717-28 PMID: 6153931
  29. Two forms of transcription factor TFIIIC in extracts from HeLa cells.
    Nucleic Acids Res. 1987 Jul 10;15(13):5031-9 PMID: 3601665
  30. A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
    Cell. 1981 Jun;24(3):809-17 PMID: 6166388
  31. TFIIIA binds to different domains of 5S RNA and the Xenopus borealis 5S RNA gene.
    Mol Cell Biol. 1987 Nov;7(11):3985-93 PMID: 3431548
  32. Onset of 5 S RNA gene regulation during Xenopus embryogenesis.
    Dev Biol. 1983 Sep;99(1):248-57 PMID: 6618002
  33. Separation of TFIIIC into two functional components by sequence specific DNA affinity chromatography.
    Nucleic Acids Res. 1987 Dec 10;15(23):9895-907 PMID: 3697084
  34. TFIIIA binds with equal affinity to somatic and major oocyte 5S RNA genes.
    Genes Dev. 1988 Feb;2(2):205-14 PMID: 3360323
  35. Differential order of replication of Xenopus laevis 5S RNA genes.
    Mol Cell Biol. 1986 Jul;6(7):2536-42 PMID: 2431292
  36. Purification and some characteristics of 5S DNA from Xenopus laevis.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3175-9 PMID: 5289255
  37. A pseudogene structure in 5S DNA of Xenopus laevis.
    Cell. 1977 Sep;12(1):109-20 PMID: 561661
  38. Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
    Mol Cell Biol. 1988 Oct;8(10):4257-69 PMID: 3185548
  39. Torsional stress induces an S1 nuclease-hypersensitive site within the promoter of the Xenopus laevis oocyte-type 5S RNA gene.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4018-22 PMID: 2987960
  40. Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
    Nat New Biol. 1973 Jan 3;241(105):7-12 PMID: 4512331
  41. Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.
    Mol Cell Biol. 1987 Oct;7(10):3503-10 PMID: 3683391
  42. Sequence heterogeneity of 5 S RNA in Xenopus laevis.
    FEBS Lett. 1972 Sep 1;25(1):13-20 PMID: 11946710
  43. Cooperative model for the binding of Xenopus transcription factor A to the 5S RNA gene.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2142-5 PMID: 6572967
  44. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  45. Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
    Cell. 1984 Jun;37(2):645-52 PMID: 6722885
  46. Mechanism of chromatin assembly in Xenopus oocytes.
    J Mol Biol. 1986 Jun 5;189(3):457-76 PMID: 3783682
  47. Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos.
    EMBO J. 1983;2(9):1613-9 PMID: 11892820
  48. Changing directions in Pol III transcription.
    Genes Dev. 1988 Apr;2(4):373-5 PMID: 3371657
  49. Resolution of human transcription factor TFIIIC into two functional components.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3585-9 PMID: 3473469
  50. Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
    Cell. 1982 Dec;31(2 Pt 1):395-405 PMID: 6297765
  51. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
    Cell. 1978 Apr;13(4):717-25 PMID: 566164
  52. The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway.
    EMBO J. 1987 Jul;6(7):1921-7 PMID: 3653074
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.2.499-514.1989
Published
1989-02-00
Pages
499-514
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362626
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.2.499-514.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2