Home LiteratureArticle Details
PMID: 3460086 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Early replication and expression of oocyte-type 5S RNA genes in a Xenopus somatic cell line carrying a translocation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 14 ·1986-07-00 ·Pages 5150-4

Guinta DR, Tso JY, Narayanswami S, Hamkalo BA, Korn LJ

Abstract

In Xenopus somatic cells, the somatic-type 5S RNA genes replicate early in S phase, bind the transcription factor TFIIIA, and are expressed; in contrast, the late replicating oocyte-type genes do not bind TFIIIA and are transcriptionally inactive. These facts support a model in which the order of replication of the somatic-type versus the oocyte-type 5S genes causes their differential expression in somatic cells due to sequestration of TFIIIA by the early-replicating somatic genes. Here we provide further evidence for the model by showing that in one Xenopus cell line in which some oocyte-type 5S genes are translocated, some oocyte-type 5S genes replicate early and are expressed.

MeSH Terms
Animals Cell Division Cell Line Female Gene Expression Regulation Interphase Models, Genetic Oocytes RNA, Ribosomal/biosynthesis,genetics Transcription Factor TFIIIA Transcription Factors/biosynthesis,genetics Translocation, Genetic Xenopus laevis/genetics,metabolism
Chemicals
RNA, Ribosomal Transcription Factor TFIIIA Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Guinta D R
Tso J Y
Narayanswami S
Hamkalo B A
Korn L J
References (29)
29 references, click to expand
  1. The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
    Cell. 1984 Jul;37(3):903-13 PMID: 6540147
  2. Changes in the rate of DNA replication fork movement during S phase in mammalian cells.
    J Mol Biol. 1975 May 15;94(2):173-81 PMID: 1170335
  3. Gene amplification in a single cell cycle in Chinese hamster ovary cells.
    J Biol Chem. 1984 Feb 10;259(3):1901-10 PMID: 6693439
  4. Chromosomal mapping of Xenopus 5S genes: somatic-type versus oocyte-type.
    Nucleic Acids Res. 1983 Apr 25;11(8):2313-23 PMID: 6687939
  5. Replication timing of genes and middle repetitive sequences.
    Science. 1984 May 18;224(4650):686-92 PMID: 6719109
  6. Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae.
    J Cell Biol. 1980 Apr;85(1):108-15 PMID: 6767729
  7. Location of the genes for 5S ribosomal RNA in Xenopus laevis.
    Chromosoma. 1973;42(2):191-203 PMID: 4726606
  8. The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
    Nature. 1981 Feb 5;289(5797):461-5 PMID: 7193290
  9. Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
    Cell. 1980 May;20(1):131-41 PMID: 6248230
  10. A positive transcription factor controls the differential expression of two 5S RNA genes.
    Cell. 1985 Oct;42(3):759-67 PMID: 4053184
  11. Changes in gene position are accompanied by a change in time of replication.
    Cell. 1984 Mar;36(3):689-96 PMID: 6421490
  12. Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
    Cell. 1982 Apr;28(4):781-91 PMID: 7201351
  13. Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1760-4 PMID: 6165017
  14. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
    Cell. 1980 Mar;19(3):717-28 PMID: 6153931
  15. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  16. The temporal order of replication of some DNA cistrons.
    Cold Spring Harb Symp Quant Biol. 1974;38:239-45 PMID: 4364782
  17. Enhancement of methotrexate resistance and dihydrofolate reductase gene amplification by treatment of mouse 3T6 cells with hydroxyurea.
    Mol Cell Biol. 1983 Jun;3(6):1097-107 PMID: 6877240
  18. CORRELATION OF GENETIC ACTIVITY, HETEROCHROMATIZATION, AND RNA METABOLISM.
    Proc Natl Acad Sci U S A. 1965 Jan;53:68-73 PMID: 14283207
  19. Differential order of replication of Xenopus laevis 5S RNA genes.
    Mol Cell Biol. 1986 Jul;6(7):2536-42 PMID: 2431292
  20. Control of 5S RNA synthesis in Xenopus laevis.
    Nature. 1976 Jun 3;261(5559):433-5 PMID: 945465
  21. Ultrastructural features of minute chromosomes in a methotrexate-resistant mouse 3T3 cell line.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1126-30 PMID: 3856243
  22. Cell cycle analysis by flow cytometry.
    Methods Enzymol. 1979;58:233-48 PMID: 423764
  23. Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
    Nat New Biol. 1973 Jan 3;241(105):7-12 PMID: 4512331
  24. Sequence heterogeneity of 5 S RNA in Xenopus laevis.
    FEBS Lett. 1972 Sep 1;25(1):13-20 PMID: 11946710
  25. Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3058-62 PMID: 6942415
  26. Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos.
    EMBO J. 1983;2(9):1613-9 PMID: 11892820
  27. Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations.
    J Histochem Cytochem. 1983 Mar;31(3):376-81 PMID: 6186731
  28. The structure and evolution of ribosomal and 5S DNAs in Xenopus laevis and Xenopus mulleri.
    Cold Spring Harb Symp Quant Biol. 1974;38:501-5 PMID: 4524770
  29. Developmental regulation of Xenopus 5S RNA genes.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:879-84 PMID: 6574872
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.14.5150
Published
1986-07-00
Pages
5150-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323908
Subset
IM
Grants
NCI NIH HHS · CA09302 · United States
NIGMS NIH HHS · GM-09947 · United States
NIGMS NIH HHS · GM23241 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.14.5150 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2