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PMID: 2724412 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The palindromic LTR-LTR junction of Moloney murine leukemia virus is not an efficient substrate for proviral integration.

Journal of virology ·Vol. 63 ·No. 6 ·1989-06-00 ·Pages 2629-37

Lobel LI, Murphy JE, Goff SP

Abstract

We generated viral constructs to test the hypothesis that the major substrate on retroviral DNA that is utilized for proviral DNA integration is the palindromic sequence, termed the LTR-LTR junction, normally present in circular molecules formed by joining the two termini of linear proviral DNA. Recombinant viral genomes were built which carried a selectable marker and an extra copy of the LTR-LTR junction from a cloned circular provirus. The junction sequence in each case was positioned such that its use during integration would lead to an easily detected, aberrantly integrated proviral DNA. Analysis of DNA from cells infected with the virus constructs showed that the introduced junction sequence is used at least 1,000-fold less efficiently than the natural sequences at the ends of the genome. This suggests that a linear or more exotic DNA intermediate is most likely the true precursor for the integration reaction.

MeSH Terms
Animals Base Sequence Blotting, Southern Cell Line Cloning, Molecular DNA, Viral/genetics Molecular Sequence Data Moloney murine leukemia virus/genetics Mutation Nucleic Acid Hybridization Plasmids Proviruses/genetics Recombination, Genetic Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lobel L I
Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Murphy J E
Goff S P
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.6.2629-2637.1989
Published
1989-06-00
Pages
2629-37
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250744
Subset
IM
Grants
NCI NIH HHS · CA 30488 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.6.2629-2637.1989 PMC Full Text View journal details
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