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PMID: 6449003 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 3932-6

Shoemaker C, Goff S, Gilboa E, Paskind M, Mitra SW, Baltimore D

Abstract

Closed circular Moloney murine leukemia virus (M-MuLV) DNA was prepared from recently infected cells and cloned in a lambda vector. Four classes of cloned M-MuLV inserts were found: Class I, full length 8.8-kilobase (kb) inserts with two tandem long terminal repeats (LTRs) of 600 base pairs; class 2, 8.2-kb inserts with a single copy of a LTR; class 3, M-MuLV DNA inserts with various portions deleted; and class 4, an 8.8-kb insert with an internal sequence inversion. Determination of nucleotide sequence at the junction between the two LTRs from a class 1 insert suggested that circularization occurred by blunt-end ligation of an 8.8-kb linear DNA. The class 4 molecule had an inversion that was flanked by inverted LTRs, each of which had lost two terminal base pairs at the inversion end points. Also, four base pairs that were present only once in standard M-MuLV DNA were duplicated at either end of the inversion. This molecule was interpreted as resulting from an integrative inversion in which M-MuLV DNA has integrated into itself. Its analysis thus provided explicit information concerning the mechanism by which retrovirus DNA integrates into host cell DNA. Models of retrovirus integration based on bacterial DNA transposition mechanisms are proposed.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Chromosome Inversion Cloning, Molecular DNA, Circular/genetics DNA, Recombinant DNA, Viral/genetics Escherichia coli/genetics Models, Genetic Moloney murine leukemia virus/genetics Recombination, Genetic
Chemicals
DNA, Circular DNA, Recombinant DNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shoemaker C
Goff S
Gilboa E
Paskind M
Mitra S W
Baltimore D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.7.3932
Published
1980-07-00
Pages
3932-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349741
Subset
IM
Grants
NCI NIH HHS · CA-14051 · United States
NCI NIH HHS · CA-26717 · United States
NCI NIH HHS · N01-CP-53562 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.7.3932 PMC Full Text View journal details
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