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PMID: 2726455 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The complete nucleotide sequence of the maize chlorotic mottle virus genome.

Nucleic acids research ·Vol. 17 ·No. 8 ·1989-04-25 ·Pages 3163-77

Nutter RC, Scheets K, Panganiban LC, Lommel SA

Abstract

The complete nucleotide sequence of the maize chlorotic mottle virus (MCMV) genome has been determined to be 4437 nucleotides. The viral genome has four long open reading frames (ORFs) which could encode polypeptides of 31.6, 50, 8.9 and 25.1 kd. If the termination codons, for the polypeptides encoded by the 50 and 8.9 kd ORFs are suppressed, readthrough products of 111 and 32.7 kd result. The 31.6 and 50 kd ORFs overlap for nearly the entire length of the 31.6 kd ORF. Striking amino acid homology has been observed between two potential polypeptides encoded by MCMV and polypeptides encoded by carnation mottle virus (CarMV) and turnip crinkle virus (TCV). The 25.1 kd ORF most likely encodes the capsid protein. The similar genome organization and amino acid sequence homology of MCMV with CarMV and TCV suggest an evolutionary relationship with these members of the carmovirus group.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Genes, Viral Molecular Sequence Data Plant Viruses/genetics RNA, Viral/genetics Restriction Mapping Viral Proteins/genetics Zea mays/microbiology
Chemicals
RNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nutter R C
Department of Plant Pathology, Oklahoma State University, Stillwater 74078-0258.
Scheets K
Panganiban L C
Lommel S A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.8.3163
Published
1989-04-25
Pages
3163-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317721
Subset
IM
Databases
GENBANK
X14736
External Links
PubMed source DOI: 10.1093/nar/17.8.3163 PMC Full Text View journal details
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