Genetic typing in a cluster of Legionella pneumophila infections.

Journal of clinical microbiology ·Vol. 27 ·No. 5 ·1989-05-00 ·Pages 1105-7

van Ketel RJ, de Wever B

Abstract

Legionella pneumophila strains isolated from six patients, three air-conditioning- and cooling tower-derived strains, and three hot water supply-derived strains were analyzed by three genetic typing methods. The results of the whole-cell DNA restriction endonuclease analysis and the restriction patterns based on genes coding for rRNA correlated with each other and demonstrated that the patient isolates were indistinguishable from the air-conditioning- and cooling tower-derived isolates but differed markedly from the hot water supply-derived isolates. The patient and air-conditioning- and cooling tower-derived strains contained plasmids of the same molecular weight; the hot water supply-derived strains were plasmidless. These results indicated that the cooling tower or the air-conditioning system was the environmental source for the examined cluster of Legionnaires disease strains.

MeSH Terms

Air Conditioning Autoradiography Cross Infection/microbiology DNA, Bacterial/analysis Genotype Hot Temperature Humans Legionella/classification,genetics Legionnaires' Disease/microbiology Netherlands Nucleic Acid Hybridization Plasmids RNA, Ribosomal/genetics Restriction Mapping Water Microbiology Water Supply

Chemicals

DNA, Bacterial RNA, Ribosomal

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

van Ketel R J

Department of Medical Microbiology, University of Amsterdam, The Netherlands.

de Wever B

References (24)

24 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.27.5.1105-1107.1989

Published

1989-05-00

Pages

1105-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC267494

Subset

External Links

PubMed source DOI: 10.1128/jcm.27.5.1105-1107.1989 PMC Full Text View journal details

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