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PMID: 3517064 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies.

Journal of clinical microbiology ·Vol. 23 ·No. 4 ·1986-04-00 ·Pages 768-71

Joly JR, McKinney RM, Tobin JO, Bibb WF, Watkins ID, Ramsay D

Abstract

A panel of monoclonal antibodies to Legionella pneumophila serogroup 1 and a subclassification scheme were developed in a collaborative project among three laboratories. The seven most useful monoclonal antibodies were selected from three previously developed panels on the basis of indirect fluorescent antibody patterns with 83 strains of L. pneumophila serogroup 1 that were obtained from widely distributed geographic locations. The isolates were divided into 10 major subgroups on the basis of reactivity patterns that can be readily reproduced in any laboratory and are not subject to major inconsistencies of interpretation of staining intensity. A standard protocol for the indirect fluorescent antibody procedure was also developed.

MeSH Terms
Antibodies, Bacterial/immunology Antibodies, Monoclonal Antigens, Bacterial/immunology Fluorescent Antibody Technique Humans Legionella/classification,immunology Legionnaires' Disease/microbiology Serotyping/standards
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Antigens, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Joly J R
McKinney R M
Tobin J O
Bibb W F
Watkins I D
Ramsay D
References (29)
29 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.23.4.768-771.1986
Published
1986-04-00
Pages
768-71
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC362834
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.23.4.768-771.1986 PMC Full Text View journal details
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