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PMID: 2762122 Published · ppublish English Journal Article

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.

Nucleic acids research ·Vol. 17 ·No. 13 ·1989-07-11 ·Pages 5115-23

Beck S, O'Keeffe T, Coull JM, Köster H

Abstract

A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.

MeSH Terms
Base Sequence DNA, Single-Stranded Indicators and Reagents Luminescent Measurements Microchemistry Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemical synthesis
Chemicals
DNA, Single-Stranded Indicators and Reagents Oligodeoxyribonucleotides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Beck S
MilliGen/Biosearch, Division of Millipore, Burlington, MA 01803.
O'Keeffe T
Coull J M
Köster H
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.13.5115
Published
1989-07-11
Pages
5115-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318099
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.13.5115 PMC Full Text View journal details
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