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PMID: 2770698 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heterogeneous genomic amplification in Streptomyces glaucescens: structure, location and junction sequence analysis.

Molecular & general genetics : MGG ·Vol. 217 ·No. 2-3 ·1989-06-00 ·Pages 437-46

Häusler A, Birch A, Krek W, Piret J, Hütter R

Abstract

Certain chromosomal markers in Streptomyces glaucescens behave unstably, being lost at high frequency as a result of extensive genomic deletion. Additionally, mutant strains possessing such deletions frequently display intense DNA amplification. With the help of a wild-type cosmid library we investigated the structure of the amplified DNA sequences (ADS) and the corresponding wild-type amplifiable units of DNA (AUD). The reiterations were heterogeneous in location, copy number and sequences involved and originated predominantly from a single 100 kb region of the chromosome called the AUD locus. All strains bearing reiterations possessed associated deletions which terminated either close to or at the ADS. The termini of four AUDs were sequenced in order to gain more knowledge about these heterogeneous amplifications. In three of the four cases investigated small, interrupted homologies were found bordering the AUDs. With the help of orthogonal-field-alternation gel electrophoresis (OFAGE) we were able to visualize a tandem reiteration of more than 1,500 kb in length.

MeSH Terms
Base Sequence Chromosome Deletion DNA, Bacterial/genetics Gene Amplification Genes, Bacterial Molecular Sequence Data Multigene Family Restriction Mapping Sequence Homology, Nucleic Acid Streptomyces/genetics
Chemicals
DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Häusler A
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Birch A
Krek W
Piret J
Hütter R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02464915
Published
1989-06-00
Pages
437-46
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02464915
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