Home LiteratureArticle Details
PMID: 27718154 Published · ppublish English Journal Article

Prognostic significance of immune cells in the tumor microenvironment and peripheral blood of gallbladder carcinoma patients.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico ·Vol. 19 ·No. 4 ·2017-04-00 ·Pages 477-488

Zhang Y, Ma C, Wang M, Hou H, Cui L, Jiang C, Sun J, Qu X

Abstract

The role of the interaction between tumor cells and inflammatory cells in gallbladder carcinoma (GBC) is unclear. Inflammatory cells exist in both the tumor immune microenvironment and the host peripheral blood circulatory system. In the current study, we examined the prognostic value of inflammatory cells in the tumor microenvironment and peripheral blood in patients with GBC. 98 patients with GBC were recruited in this retrospective study. Using immunohistochemistry, we examined tumor-infiltrating CD3+ generic T-cells, CD8+ cytotoxic T-cells, CD45RO+ memory T-cells, and CD15+ neutrophils. Peripheral venous blood samples were also collected, and absolute neutrophil count (ANC), absolute lymphocyte count (ALC) and neutrophil/lymphocyte ratio (NLR) were measured. The relationships between these variables and patient outcome were evaluated. Survival analysis revealed that the density of CD3+ cell infiltrates in the tumor microenvironment was positively correlated with overall survival (OS) and the density of CD15+ cell infiltrates was negatively correlated with the OS. The combined analysis showed that a high density of CD3+ cell infiltrates combined with a low density of CD15+ cell infiltrates was an independent prognostic factor for GBC. In peripheral blood, survival analysis suggested that ANC and NLR were negatively correlated, while ALC was positively correlated with OS. Multivariate survival analysis showed that NLR was an independent prognostic factor for gallbladder cancer prognosis. The results indicate that the combination of high density of CD3+ cell infiltrates combined with a low density of CD15+ cell infiltrates in tumor samples and pretreatment peripheral blood NLR were independent prognostic factors in patients with GBC.

Keywords
Gallbladder carcinoma Immunohistochemistry Neutrophil/lymphocyte ratio Tumor microenvironment Tumor-infiltrating lymphocytes
MeSH Terms
Adult Aged Aged, 80 and over Carcinoma, Squamous Cell/blood,immunology,secondary Female Follow-Up Studies Gallbladder Neoplasms/blood,immunology,pathology Humans Immunoenzyme Techniques Inflammation/blood,immunology,pathology Lymphatic Metastasis Lymphocytes, Tumor-Infiltrating/immunology Male Middle Aged Neoplasm Grading Neoplasm Staging Neutrophils/immunology Prognosis Retrospective Studies Survival Rate Tumor Microenvironment/immunology
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Zhang Y
Department of Oncology, School of Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266011, Shandong, People's Republic of China.
Ma C
Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, 250012, Shandong, People's Republic of China.
Wang M
Department of Oncology, School of Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266011, Shandong, People's Republic of China.
Hou H
Department of Oncology, School of Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266011, Shandong, People's Republic of China.
Cui L
Department of Pathology, School of Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266011, Shandong, People's Republic of China.
Jiang C
Department of Pathology, School of Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266011, Shandong, People's Republic of China.
Sun J
Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, 250012, Shandong, People's Republic of China.
Qu X ORCID
Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, 250012, Shandong, People's Republic of China. [email protected].
References (29)
29 references, click to expand
  1. Effector memory T cells, early metastasis, and survival in colorectal cancer.
    N Engl J Med. 2005 Dec 22;353(25):2654-66 PMID: 16371631
  2. Surgery for gallbladder cancer: a population-based analysis.
    J Am Coll Surg. 2008 Sep;207(3):371-82 PMID: 18722943
  3. Prognostic significance of preoperative neutrophil/lymphocyte ratio and platelet/lymphocyte ratio in patients with gallbladder carcinoma.
    Clin Transl Oncol. 2015 Oct;17(10):810-8 PMID: 26077119
  4. Gallbladder cancer: differences in presentation, surgical treatment, and survival in patients treated at centers in three countries.
    J Am Coll Surg. 2011 Jan;212(1):50-61 PMID: 21075015
  5. Clinical evaluation of systemic and local immune responses in cancer: time for integration.
    Cancer Immunol Immunother. 2014 Jan;63(1):45-57 PMID: 24100804
  6. The in situ local immune response, tumour senescence and proliferation in colorectal cancer.
    Br J Cancer. 2013 Oct 15;109(8):2207-16 PMID: 24022192
  7. The prognostic value of tumor-infiltrating neutrophils in gastric adenocarcinoma after resection.
    PLoS One. 2012;7(3):e33655 PMID: 22442706
  8. Tumour-associated CD66b+ neutrophil count is an independent prognostic factor for recurrence in localised cervical cancer.
    Br J Cancer. 2013 May 28;108(10):2116-22 PMID: 23591202
  9. Neutrophils and immunity: challenges and opportunities.
    Nat Rev Immunol. 2006 Mar;6(3):173-82 PMID: 16498448
  10. Increased intratumoral neutrophil in colorectal carcinomas correlates closely with malignant phenotype and predicts patients' adverse prognosis.
    PLoS One. 2012;7(1):e30806 PMID: 22295111
  11. Immune cell infiltration as an indicator of the immune microenvironment of pancreatic cancer.
    Br J Cancer. 2013 Mar 5;108(4):914-23 PMID: 23385730
  12. Clinical significance of immune cell infiltration within gallbladder cancer.
    Br J Cancer. 2003 Nov 3;89(9):1736-42 PMID: 14583778
  13. Usefulness of the neutrophil-to-lymphocyte ratio in predicting short- and long-term mortality in breast cancer patients.
    Ann Surg Oncol. 2012 Jan;19(1):217-24 PMID: 21638095
  14. Cancer immunoediting: integrating immunity's roles in cancer suppression and promotion.
    Science. 2011 Mar 25;331(6024):1565-70 PMID: 21436444
  15. Hepatocyte growth factor production by neutrophils infiltrating bronchioloalveolar subtype pulmonary adenocarcinoma: role in tumor progression and death.
    Cancer Res. 2003 Mar 15;63(6):1405-12 PMID: 12649206
  16. Immunomonitoring and prognostic relevance of neutrophils in clinical trials.
    Semin Cancer Biol. 2013 Jun;23(3):200-7 PMID: 23403174
  17. Preoperative platelet to lymphocyte ratio predicts outcome of patients with pancreatic ductal adenocarcinoma after pancreatic resection.
    Surgery. 2015 Aug;158(2):360-5 PMID: 26032829
  18. The Glasgow Prognostic Score, an inflammation based prognostic score, predicts survival in patients with hepatocellular carcinoma.
    BMC Cancer. 2013 Feb 02;13:52 PMID: 23374755
  19. High number of CD45RO+ tumor infiltrating lymphocytes is an independent prognostic factor in non-metastasized (stage I-IIA) esophageal adenocarcinoma.
    BMC Cancer. 2010 Nov 05;10:608 PMID: 21054833
  20. Evaluation of two inflammation-based prognostic scores in patients with resectable gallbladder carcinoma.
    Ann Surg Oncol. 2014 Feb;21(2):449-57 PMID: 24081806
  21. Detailed analysis of inflammatory cell infiltration in colorectal cancer.
    Br J Cancer. 2013 Oct 1;109(7):1839-47 PMID: 24008661
  22. Prognostic significance of CD45RO+ memory T cells in renal cell carcinoma.
    Br J Cancer. 2011 Oct 11;105(8):1191-6 PMID: 21934683
  23. The clinical significance of tumor-infiltrating neutrophils and neutrophil-to-CD8+ lymphocyte ratio in patients with resectable esophageal squamous cell carcinoma.
    J Transl Med. 2014 Jan 07;12:7 PMID: 24397835
  24. Neutrophils and angiogenesis: potential initiators of the angiogenic cascade.
    Chem Immunol Allergy. 2003;83:167-81 PMID: 12947984
  25. Gastric carcinoma with prominent neutrophil infiltration.
    Histopathology. 2000 Sep;37(3):289-90 PMID: 10971710
  26. Pretreatment levels of peripheral neutrophils and leukocytes as independent predictors of overall survival in patients with American Joint Committee on Cancer Stage IV Melanoma: results of the EORTC 18951 Biochemotherapy Trial.
    J Clin Oncol. 2007 Apr 20;25(12):1562-9 PMID: 17443000
  27. Presence of intratumoral neutrophils is an independent prognostic factor in localized renal cell carcinoma.
    J Clin Oncol. 2009 Oct 1;27(28):4709-17 PMID: 19720929
  28. CXCL17 and ICAM2 are associated with a potential anti-tumor immune response in early intraepithelial stages of human pancreatic carcinogenesis.
    Gastroenterology. 2011 Jan;140(1):310-21 PMID: 20955708
  29. Prognostic significance of activated CD8(+) T cell infiltrations within esophageal carcinomas.
    Cancer Res. 2001 May 15;61(10):3932-6 PMID: 11358808
Article Info
Journal
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
Abbr.
Clin Transl Oncol
ISSN
1699-3055
DOI
10.1007/s12094-016-1553-6
Email
[email protected]
Published
2017-04-00
Epub
2016-00-07
Pages
477-488
Language
English
Region
Italy
NLM ID
101247119
Subset
IM
External Links
PubMed source DOI: 10.1007/s12094-016-1553-6 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2