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PMID: 2773938 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

High-resolution analysis of a hypervariable region in the human apolipoprotein B gene.

American journal of human genetics ·Vol. 45 ·No. 3 ·1989-09-00 ·Pages 458-64

Ludwig EH, Friedl W, McCarthy BJ

Abstract

A hypervariable region occurs immediately 3' of the human apolipoprotein B gene. Several allelic variants of this tandemly repeated sequence can be resolved by genomic blotting. Higher resolution among size variants may be obtained by polymerase-chain-reaction amplification of this region followed by electrophoresis in a denaturing acrylamide gel. Fourteen different alleles containing 25-52 repeats of the basic 15-bp unit were distinguished in a population study of 318 unrelated individuals. This approach should be applicable to pedigree and linkage analysis with the apolipoprotein B gene or other tandemly repeated sequence elements.

MeSH Terms
Alleles Apolipoproteins B/genetics Electrophoresis, Polyacrylamide Gel Gene Amplification Gene Frequency Genetic Variation Humans Molecular Sequence Data Oligonucleotide Probes Plasmids Repetitive Sequences, Nucleic Acid
Chemicals
Apolipoproteins B Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ludwig E H
Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140-0608.
Friedl W
McCarthy B J
References (23)
23 references, click to expand
  1. Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells.
    Nucleic Acids Res. 1988 Dec 9;16(23):10953-71 PMID: 3205737
  2. Familial defective apolipoprotein B-100: enhanced binding of monoclonal antibody MB47 to abnormal low density lipoproteins.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9758-62 PMID: 3200853
  3. Association between a specific apolipoprotein B mutation and familial defective apolipoprotein B-100.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):587-91 PMID: 2563166
  4. Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase.
    Biotechniques. 1988 Jun;6(6):544-6, 549 PMID: 3273187
  5. The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences.
    Nature. 1982 Jan 7;295(5844):31-5 PMID: 7035959
  6. Hypervariable 'minisatellite' regions in human DNA.
    Nature. 1985 Mar 7-13;314(6006):67-73 PMID: 3856104
  7. A highly polymorphic region 3' to the human type II collagen gene.
    Nucleic Acids Res. 1985 Jul 11;13(13):4613-22 PMID: 4022769
  8. Molecular characterisation of a hypervariable region downstream of the human alpha-globin gene cluster.
    EMBO J. 1986 Aug;5(8):1857-63 PMID: 3019666
  9. Structure of the human apolipoprotein B gene.
    J Biol Chem. 1986 Nov 25;261(33):15364-7 PMID: 2946672
  10. Apolipoprotein B-gene DNA polymorphisms associated with myocardial infarction.
    N Engl J Med. 1986 Dec 11;315(24):1509-15 PMID: 3024002
  11. A hypervariable region 3' to the human apolipoprotein B gene.
    Nucleic Acids Res. 1986 Nov 25;14(22):9215-6 PMID: 3786150
  12. Lipoprotein mutations in pigs are associated with elevated plasma cholesterol and atherosclerosis.
    Science. 1986 Dec 19;234(4783):1573-7 PMID: 3787263
  13. DNA polymorphism at the apolipoprotein B locus is associated with lipoprotein level.
    Clin Genet. 1986 Dec;30(6):515-20 PMID: 3502691
  14. Variable number of tandem repeat (VNTR) markers for human gene mapping.
    Science. 1987 Mar 27;235(4796):1616-22 PMID: 3029872
  15. A unique AT-rich hypervariable minisatellite 3' to the ApoB gene defines a high information restriction fragment length polymorphism.
    J Biol Chem. 1987 Jul 5;262(19):8952-5 PMID: 2885324
  16. DNA sequence of the human apolipoprotein B gene.
    DNA. 1987 Aug;6(4):363-72 PMID: 3652907
  17. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  18. Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA.
    Nature. 1988 Mar 17;332(6161):278-81 PMID: 3347271
  19. Characterization of genetic markers in the 3' end of the apo B gene and their use in family and population studies.
    Atherosclerosis. 1988 Jan;69(1):39-49 PMID: 2895657
  20. Genetic factors affecting blood lipoproteins: the candidate gene approach.
    J Lipid Res. 1988 Apr;29(4):397-429 PMID: 3292686
  21. A new hypervariable marker for the human alpha-globin gene cluster.
    Am J Hum Genet. 1988 Sep;43(3):249-56 PMID: 2901223
  22. Intrinsic polymorphism of variable number tandem repeat loci in the human genome.
    Nucleic Acids Res. 1988 Sep 12;16(17):8487-96 PMID: 2901724
  23. Rapid typing of tandemly repeated hypervariable loci by the polymerase chain reaction: application to the apolipoprotein B 3' hypervariable region.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):212-6 PMID: 2911570
Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1989-09-00
Pages
458-64
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1683401
Subset
IM
Grants
NHLBI NIH HHS · HL 38781 · United States
External Links
PubMed source PMC Full Text View journal details
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