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PMID: 3205737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells.

Nucleic acids research ·Vol. 16 ·No. 23 ·1988-12-09 ·Pages 10953-71

Jeffreys AJ, Wilson V, Neumann R, Keyte J

Abstract

Hypervariable minisatellites can be amplified from human DNA by the polymerase chain reaction, using primers from DNA flanking the minisatellite to amplify the entire block of tandem repeat units. Minisatellite alleles up to 5-10 kb long can be faithfully amplified. At least six minisatellite loci can be co-amplified from the same DNA sample and simultaneously detected to provide a reproducible and highly variable DNA fingerprint which can be obtained from nanogram quantities of human DNA. The polymerase chain reaction can also be used to analyse single target minisatellite molecules and single human cells, despite the appearance of spurious PCR products from some hypervariable loci. DNA fingerprinting at the level of one or a few cells therefore appears possible.

MeSH Terms

Alleles Blotting, Southern DNA, Satellite/isolation & purification,metabolism DNA-Directed DNA Polymerase/metabolism Gene Amplification Genotype Humans Lymphocytes/metabolism Nucleic Acid Hybridization Oligonucleotide Probes

Chemicals

DNA, Satellite Oligonucleotide Probes DNA-Directed DNA Polymerase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jeffreys A J

Department of Genetics, University of Leicester, UK.

Wilson V

Neumann R

Keyte J

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28 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.23.10953

Published

1988-12-09

Pages

10953-71

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338990

Subset

External Links

PubMed source DOI: 10.1093/nar/16.23.10953 PMC Full Text View journal details

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Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells.

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