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PMID: 2423969 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning a selected fragment from a human DNA 'fingerprint': isolation of an extremely polymorphic minisatellite.

Nucleic acids research ·Vol. 14 ·No. 11 ·1986-06-11 ·Pages 4605-16

Wong Z, Wilson V, Jeffreys AJ, Thein SL

Abstract

A large hypervariable DNA fragment from a human DNA fingerprint was purified by preparative gel electrophoresis and molecular cloning. The cloned fragment contained a 6.3 kb long minisatellite consisting of multiple copies of a 37 bp repeat unit. Each repeat contained an 11 bp copy of the "core" sequences, a putative recombination signal in human DNA. The cloned minisatellite hybridized to a single locus in the human genome. This locus is extremely polymorphic, with at least 77 different alleles containing 14 to 525 repeat units per allele being resolved in a sample of 79 individuals. All alleles except the shortest are rare and the resulting heterozygosity is very high (approximately 97%). Cloned minisatellites should therefore provide a panel of extremely informative locus-specific probes ideal for linkage analysis in man.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular/methods DNA, Satellite/genetics Fetal Hemoglobin/genetics Gene Expression Regulation Genetic Linkage Humans Polymorphism, Genetic Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Satellite Fetal Hemoglobin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wong Z
Wilson V
Jeffreys A J
Thein S L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.11.4605
Published
1986-06-11
Pages
4605-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311468
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.11.4605 PMC Full Text View journal details
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