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PMID: 2774708 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Does the bacteremia observed in hemorrhagic shock have clinical significance? A study in germ-free animals.

Annals of surgery ·Vol. 210 ·No. 3 ·1989-09-00 ·Pages 342-5; discussion 346-7

Rush BF, Redan JA, Flanagan JJ, Heneghan JB, Hsieh J, Murphy TF, Smith S, Machiedo GW

Abstract

We have recently reported the rapid appearance of bacteria and endotoxin in the blood of rats and of trauma patients in the course of 30 minutes to 2 hours of hemorrhagic shock. The current study was designed to determine the effect of this bacteremia and endotoxemia on survival. Thirty-three conventional (C:group 1) and 36 germ-free (GF:group 2) Sprague Dawley rats were subjected to our previously described model of treated hemorrhagic shock. Survival in the GF group was significantly better than the C group at 24, 48, and 72 hours after shock. Endotoxin levels were elevated in 88% of C group during shock and in 28% of GF group. The gut of the GF animal contains endotoxin (26 ng/gm of stool) as does the sterile food supply (393 ng/gm of rat chow).

MeSH Terms
Animals Endotoxins/analysis,blood Feces/analysis Germ-Free Life Rats Rats, Inbred Strains Reference Values Sepsis/etiology Shock, Hemorrhagic/complications,metabolism,mortality Time Factors
Chemicals
Endotoxins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Rush B F
Department of Surgery, New Jersey Medical School, Newark 07103.
Redan J A
Flanagan J J
Heneghan J B
Hsieh J
Murphy T F
Smith S
Machiedo G W
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Article Info
Journal
Annals of surgery
Abbr.
Ann Surg
ISSN
0003-4932
DOI
10.1097/00000658-198909000-00010
Published
1989-09-00
Pages
342-5; discussion 346-7
Language
English
Region
United States
NLM ID
0372354
PMCID
PMC1358000
Subset
IM
Grants
NIGMS NIH HHS · GM37060-03 · United States
External Links
PubMed source DOI: 10.1097/00000658-198909000-00010 PMC Full Text View journal details
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