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PMID: 2779518 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression.

Molecular & general genetics : MGG ·Vol. 218 ·No. 2 ·1989-08-00 ·Pages 330-9

Eikmanns BJ, Follettie MT, Griot MU, Sinskey AJ

Abstract

The ppc gene of Corynebacterium glutamicum encoding phosphoenolpyruvate (PEP) carboxylase was isolated by complementation of a ppc mutant of Escherichia coli using a cosmid gene bank of chromosomal C. glutamicum DNA. By subsequent subcloning into the plasmid pUC8 and deletion analysis, the ppc gene could be located on a 3.3 kb SalI fragment. This fragment was able to complement the E. coli ppc mutant and conferred PEP carboxylase activity to the mutant. The complete nucleotide sequence of the ppc gene including 5' and 3' flanking regions has been determined and the primary structure of PEP carboxylase was deduced. The sequence predicts a 919 residue protein product (molecular weight of 103 154) which shows 34% similarity with the respective E. coli enzyme.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Carboxy-Lyases/genetics Cloning, Molecular Corynebacterium/enzymology,genetics DNA, Bacterial Genes, Bacterial Genotype Molecular Sequence Data Phosphoenolpyruvate Carboxylase/biosynthesis,genetics Plasmids Restriction Mapping Transformation, Genetic
Chemicals
DNA, Bacterial Carboxy-Lyases Phosphoenolpyruvate Carboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Eikmanns B J
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Follettie M T
Griot M U
Sinskey A J
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331286
Published
1989-08-00
Pages
330-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X14234
External Links
PubMed source DOI: 10.1007/BF00331286
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