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PMID: 2779565 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Repair of a synthetic abasic site in DNA in a Xenopus laevis oocyte extract.

Molecular and cellular biology ·Vol. 9 ·No. 9 ·1989-09-00 ·Pages 3750-7

Matsumoto Y, Bogenhagen DF

Abstract

Covalently closed circular DNA containing a synthetic analog of an abasic site at a unique position was used as a substrate to study DNA repair. Incubation of this DNA in Xenopus laevis oocyte extracts resulted in rapid cleavage of the DNA at the abasic site by a class II apurinic-apyrimidinic endonuclease, followed by complete repair within 40 min. Nicked circular DNAs persisted for several minutes before repair by an ATP-dependent DNA synthesis reaction. The repair-related DNA synthesis was localized within 3 or 4 nucleotides surrounding the abasic site. These results are consistent with the short-patch repair reported for DNA damage at heterogeneous sites in human cells (J. D. Regan and R. B. Setlow, Cancer Res. 34:3318-3325, 1974).

MeSH Terms
Animals Base Sequence Binding Sites DNA/biosynthesis DNA Damage DNA Repair DNA, Circular/metabolism Female Furans In Vitro Techniques Molecular Sequence Data Oocytes/metabolism Xenopus laevis/metabolism
Chemicals
DNA, Circular Furans tetrahydrofuran DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Matsumoto Y
Department of Pharmacological Sciences, State University of New York, Stony Brook 11794.
Bogenhagen D F
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27 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.9.3750-3757.1989
Published
1989-09-00
Pages
3750-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362436
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.9.3750-3757.1989 PMC Full Text View journal details
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