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PMID: 2783921 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of a mutant of Haemophilus influenzae type b deficient in outer membrane protein P1.

Infection and immunity ·Vol. 57 ·No. 3 ·1989-03-00 ·Pages 1002-4

Munson R, Hunt A

Abstract

The gene for outer membrane protein P1 of Haemophilus influenzae type b has been previously cloned and expressed in Escherichia coli. To investigate the physiologic role of the P1 protein, the cloned P1 gene was insertionally inactivated with the Tn5 derivative Tn5tac1, and an isogenic P1-deficient Haemophilus mutant was then generated by transformation with linearized plasmid DNA containing the insertionally inactivated gene. The P1-deficient strain grew normally in vitro and induced bacteremia in the infant rat model.

MeSH Terms

Bacterial Outer Membrane Proteins/genetics Blotting, Southern DNA, Bacterial/genetics Genes, Bacterial Haemophilus influenzae/genetics Molecular Weight Mutation

Chemicals

Bacterial Outer Membrane Proteins DNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Munson R

Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.

Hunt A

References (12)

12 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.57.3.1002-1004.1989

Published

1989-03-00

Pages

1002-4

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC313214

Subset

Grants

NIAID NIH HHS · AI 07163 · United States

NIAID NIH HHS · R01 AI 17572 · United States

External Links

PubMed source DOI: 10.1128/iai.57.3.1002-1004.1989 PMC Full Text View journal details

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Isolation and characterization of a mutant of Haemophilus influenzae type b deficient in outer membrane protein P1.

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