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PMID: 2784565 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cDNA sequences of two inducible T-cell genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 6 ·1989-03-00 ·Pages 1963-7

Kwon BS, Weissman SM

Abstract

We have previously described a set of human T-lymphocyte-specific cDNA clones isolated by a modified differential screening procedure. Apparent full-length cDNAs containing the sequences of 14 of the 16 initial isolates were sequenced and were found to represent five different species of mRNA; three of the five species were identical to previously reported cDNA sequences of preproenkephalin, T-cell-replacing factor, and a serine esterase, respectively. The other two species, 4-1BB and L2G25B, were inducible sequences found in mRNA from both a cytolytic T-lymphocyte and a helper T-lymphocyte clone and were not previously described in T-cell mRNA; these mRNA sequences encode peptides of 256 and 92 amino acids, respectively. Both peptides contain putative leader sequences. The protein encoded by 4-1BB also has a potential membrane anchor segment and other features also seen in known receptor proteins.

MeSH Terms
Adjuvants, Immunologic Amino Acid Sequence Animals Base Sequence Biological Evolution Cell Line DNA/genetics Enkephalins/genetics Esterases/genetics Humans Interleukin-5 Interleukins/genetics Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Nucleic Acid Hybridization Protein Precursors/genetics RNA, Messenger/genetics Sequence Homology, Nucleic Acid T-Lymphocytes/metabolism
Chemicals
Adjuvants, Immunologic Enkephalins Interleukin-5 Interleukins Protein Precursors RNA, Messenger DNA preproenkephalin Esterases serine esterase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kwon B S
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.
Weissman S M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.6.1963
Published
1989-03-00
Pages
1963-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286825
Subset
IM
Grants
NIAID NIH HHS · AI23058 · United States
NIAID NIH HHS · AI28175 · United States
Databases
GENBANK
J04491, J04492
External Links
PubMed source DOI: 10.1073/pnas.86.6.1963 PMC Full Text View journal details
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