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PMID: 2788410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The phototrophic bacterium Rhodopseudomonas capsulata sp108 encodes an indigenous class A beta-lactamase.

The Biochemical journal ·Vol. 260 ·No. 3 ·1989-06-15 ·Pages 803-12

Campbell JI, Scahill S, Gibson T, Ambler RP

Abstract

The nucleotide sequence of a 2.37 kb DNA fragment derived from cloning a total DNA digest of Rhodopseudomonas capsulata sp108 was determined. The DNA codes for a beta-lactamase, a protein showing sequence similarity to the ampR protein of Enterobacter cloacae and an unidentified open reading frame. Hybridization experiments with a probe carrying DNA from within the beta-lactamase gene suggests a chromosomal location for the coding sequences in strain sp108 and in sp109, a penicillin-sensitive revertant of sp108 in which the enzyme is not inducible. A protein-sequence comparison of the deduced amino acid sequence of the Rps. capsulata beta-lactamase indicates that it is a Class A enzyme and that its sequence can be aligned with those of the characterized beta-lactamases from Staphylococcus aureus, Bacillus licheniformis and the Escherichia coli plasmid (R-TEM enzyme), with only a few insertions or deletions. The corresponding DNA sequence is, however, characteristically rhodopseudomonad, suggesting that it is not a recently transposed gene.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial Genes, Bacterial Molecular Sequence Data Rhodopseudomonas/enzymology,genetics beta-Lactamases/classification,genetics
Chemicals
DNA, Bacterial beta-Lactamases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Campbell J I
Department of Molecular Biology, University of Edinburgh, Scotland.
Scahill S
Gibson T
Ambler R P
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2600803
Published
1989-06-15
Pages
803-12
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1138748
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2600803 PMC Full Text View journal details
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