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PMID: 3032094 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular genetic analysis of cephalosporinase production and its role in beta-lactam resistance in clinical isolates of Enterobacter cloacae.

Antimicrobial agents and chemotherapy ·Vol. 31 ·No. 2 ·1987-02-00 ·Pages 295-9

Nicolas MH, Honore N, Jarlier V, Philippon A, Cole ST

Abstract

Two strains of Enterobacter cloacae were isolated from a patient before (strain MHN1) and during (strain MHN2) treatment with moxalactam and gentamicin. Strain MHN1 exhibited inducible ampC cephalosporinase production. In contrast, strain MHN2 expressed the enzyme constitutively at a 3,000-fold higher level. With the Escherichia coli ampC gene as a hybridization probe it was shown that the genomic arrangement of the ampC region was the same in both strains. To gain more insight into regulatory phenomena, the ampC genes were cloned, and their expression was studied in E. coli K-12. The ampC gene from MHN1 behaved normally and conferred inducible beta-lactam resistance. A regulatory region of at least 800 base pairs involved in controlling repression-induction was located immediately upstream of ampC. Surprisingly, when present in E. coli the ampC gene from MHN2 no longer overproduced the cephalosporinase, and inducible expression was observed. This indicates that in MHN2 stable cephalosporinase overproduction is controlled by another factor which is not linked to the ampC gene.

MeSH Terms
Anti-Bacterial Agents/pharmacology Cephalosporinase/biosynthesis,genetics Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/analysis Drug Resistance, Microbial Enterobacter/drug effects,enzymology,genetics Enterobacteriaceae/genetics Enzyme Induction Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Humans Mutation Nucleic Acid Hybridization Plasmids beta-Lactamases/genetics beta-Lactams
Chemicals
Anti-Bacterial Agents DNA, Bacterial beta-Lactams DNA Restriction Enzymes Cephalosporinase beta-Lactamases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nicolas M H
Honore N
Jarlier V
Philippon A
Cole S T
References (30)
30 references, click to expand
  1. Inducible beta-lactamase in Enterobacter.
    J Gen Microbiol. 1967 Nov;49(2):277-85 PMID: 5183475
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. The use of analytical isoelectric focusing for detection and identification of beta-lactamases.
    J Gen Microbiol. 1975 May;88(1):169-78 PMID: 807678
  4. Susceptibility of Enterobacter to cefamandole: evidence for a high mutation rate to resistance.
    Antimicrob Agents Chemother. 1976 Jun;9(6):970-4 PMID: 938026
  5. The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics.
    J Antimicrob Chemother. 1976 Jun;2(2):115-57 PMID: 783110
  6. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  7. Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions.
    J Bacteriol. 1977 Dec;132(3):912-22 PMID: 336612
  8. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  9. Isolation and characterization of DNA repetitions carrying the chromosomal beta-lactamase gene of Escherichia coli K-12.
    Mol Gen Genet. 1979 Jun 7;173(2):115-25 PMID: 386030
  10. Beta-lactam resistance in clinical isolates of Escherichia coli caused by elevated production of the ampC-mediated chromosomal beta-lactamase.
    Antimicrob Agents Chemother. 1979 Oct;16(4):427-33 PMID: 391149
  11. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.
    Gene. 1980 Aug;10(3):249-59 PMID: 6254843
  12. ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4897-901 PMID: 6795623
  13. Cloning and expression in Escherichia coli K-12 of the genes for major outer membrane protein OmpA from Shigella dysenteriae, Enterobacter aerogenes, and Serratia marcescens.
    J Bacteriol. 1982 Jan;149(1):145-50 PMID: 7033204
  14. Nucleotide sequence coding for the flavoprotein subunit of the fumarate reductase of Escherichia coli.
    Eur J Biochem. 1982 Mar 1;122(3):479-84 PMID: 7037404
  15. Overlap between ampC and frd operons on the Escherichia coli chromosome.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1111-5 PMID: 7041115
  16. Mutational enzymatic resistance of Enterobacter species to beta-lactam antibiotics.
    Antimicrob Agents Chemother. 1982 Apr;21(4):655-60 PMID: 6979311
  17. Trapping of nonhydrolyzable cephalosporins by cephalosporinases in Enterobacter cloacae and Pseudomonas aeruginosa as a possible resistance mechanism.
    Antimicrob Agents Chemother. 1982 May;21(5):711-7 PMID: 6808912
  18. Characterization of beta-lactamase induction in Enterobacter cloacae.
    Antimicrob Agents Chemother. 1983 Jan;23(1):91-7 PMID: 6402977
  19. Insertion of IS2 creates a novel ampC promoter in Escherichia coli.
    Cell. 1983 Mar;32(3):809-16 PMID: 6187472
  20. Broad-spectrum beta-lactam resistance in Enterobacter: emergence during treatment and mechanisms of resistance.
    J Antimicrob Chemother. 1983 Apr;11(4):299-310 PMID: 6602122
  21. Comparison of the overlapping frd and ampC operons of Escherichia coli with the corresponding DNA sequences in other gram-negative bacteria.
    J Bacteriol. 1983 Sep;155(3):1297-305 PMID: 6350266
  22. Emergence of resistance during therapy with the newer beta-lactam antibiotics: role of inducible beta-lactamases and implications for the future.
    Rev Infect Dis. 1983 Jul-Aug;5(4):639-48 PMID: 6353526
  23. Overlapping genes.
    Annu Rev Genet. 1983;17:499-525 PMID: 6198955
  24. Identification of a novel ampC beta-lactamase promoter in a clinical isolate of Escherichia coli.
    EMBO J. 1982;1(11):1411-6 PMID: 6765197
  25. [Enterobacter cloacae. In vivo emergence of a variant resistant to new beta-lactams during treatment with lamoxactam-gentamicin].
    Pathol Biol (Paris). 1984 May;32(5):399-403 PMID: 6739148
  26. Epidemiology of enterobacteria resistant to cefotaxime in hospital.
    J Antimicrob Chemother. 1984 Sep;14 Suppl B:53-7 PMID: 6094463
  27. Survey of the phenotypes of susceptibility to beta-lactams in Enterobacteriaceae at the Pitie-Salpetriere Hospital.
    J Antimicrob Chemother. 1984 Sep;14 Suppl B:59-65 PMID: 6501135
  28. Regulatory components in Citrobacter freundii ampC beta-lactamase induction.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4620-4 PMID: 2991883
  29. Gram-negative bacilli resistant to third-generation cephalosporins: beta-lactamase characterization and susceptibility to Sch 34343.
    J Antimicrob Chemother. 1985 Jun;15 Suppl C:119-32 PMID: 3875602
  30. Computerized microacidimetric determination of beta lactamase Michaelis-Menten constants.
    FEBS Lett. 1973 Jun 15;33(1):42-4 PMID: 4198636
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.31.2.295
Published
1987-02-00
Pages
295-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC174709
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.31.2.295 PMC Full Text View journal details
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