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PMID: 278967 Published · ppublish English Journal Article

Frequency distribution of mRNA and pre-mRNA in growing and differentiated Friend cells.

Nucleic acids research ·Vol. 5 ·No. 8 ·1978-08-00 ·Pages 3013-32

Mauron A, Spohr G

Abstract

The frequency distribution of poly(A)+-mRNA in growing and in differentiated Friend cells has been measured by mRNA-cDNA hybridization and their differences established by heterologous hybridization of mRNA of one type and cDNA of the other. It was shown that induction of Friend cells involves an increase in abundance of a small number of mRNAs, while no specific pattern of messenger disappearance could be detected. The frequency distribution of pre-mRNA was determined by hybridizing nuclear RNA with the cDNA probes complementary to mRNA. In uninduced Friend cells, it was shown that most precursor messenger sequences are present at a single frequency of about 3 molecules per nucleus, independently of their final frequency in polysomal mRNA. In induced Friend cells, the frequency distribution of pre-mRNA is more heterogeneous and correlated to some extent with the corresponding mRNA frequency distribution.

MeSH Terms
Animals Cell Differentiation Cell Nucleus/metabolism Erythropoiesis Friend murine leukemia virus Kinetics Leukemia, Erythroblastic, Acute/metabolism Leukemia, Experimental/metabolism Nucleic Acid Hybridization Nucleic Acid Precursors/metabolism Poly A/metabolism Polyribosomes/metabolism RNA, Messenger/metabolism
Chemicals
Nucleic Acid Precursors RNA, Messenger Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mauron A
Spohr G
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.8.3013
Published
1978-08-00
Pages
3013-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342223
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.8.3013 PMC Full Text View journal details
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