Home LiteratureArticle Details
PMID: 2807997 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Light and electron microscopic detection of (3 Gal beta 1,4 GlcNAc beta 1) sequences in asparagine-linked oligosaccharides with the Datura stramonium lectin.

Histochemistry ·Vol. 92 ·No. 6 ·1989-00-00 ·Pages 515-22

Egea G, Goldstein IJ, Roth J

Abstract

The Datura stramonium lectin recognizes with high affinity the disaccharide N-acetyllactosamine (Gal beta 1,4 GlcNAc). We have developed a highly specific cytochemical affinity technique in which an ovomucoid-gold complex serves as second step reagent for the visualization of this lectin bound to reactive sequences present in tissue sections. The lectin binding sites were detected in semithin and ultrathin sections of aldehyde-fixed and low temperature Lowicryl K4M embedded tissues. For light microscopical labeling the photochemical silver reaction for signal amplification was required. The application of this technique for the detection of N-acetyllactosamine containing asparagine-linked oligosaccharides in various intracellular organelles and the plasma membrane is demonstrated.

MeSH Terms
Acetylgalactosamine/metabolism Animals Asparagine/metabolism Duodenum/cytology,metabolism Galactosamine/analogs & derivatives Immunohistochemistry/methods Lectins Oligosaccharides/metabolism Plant Lectins Rats Submandibular Gland/cytology,metabolism
Chemicals
Datura stramonium lectin Lectins Oligosaccharides Plant Lectins Asparagine Galactosamine Acetylgalactosamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Egea G
Interdepartmental Electron Microscopy, University of Basel, Switzerland.
Goldstein I J
Roth J
References (25)
25 references, click to expand
  1. Histochemical localization of galactose-containing glycoconjugates in sensory neurons and their processes in the central and peripheral nervous system of the rat.
    J Histochem Cytochem. 1985 Oct;33(10):1042-52 PMID: 4045182
  2. Cytochemical localization of terminal N-acetyl-D-galactosamine residues in cellular compartments of intestinal goblet cells: implications for the topology of O-glycosylation.
    J Cell Biol. 1984 Feb;98(2):399-406 PMID: 6693488
  3. Light microscopical detection of antigens and lectin binding sites with gold-labelled reagents on semi-thin Lowicryl K4M sections: usefulness of the photochemical silver reaction for signal amplification.
    Histochem J. 1987 Apr;19(4):235-45 PMID: 2439481
  4. RCA I-binding patterns of the Golgi apparatus.
    Eur J Cell Biol. 1986 Aug;41(2):270-8 PMID: 3758085
  5. Glycosylation of developing human glomeruli: lectin binding sites during cell induction and maturation.
    J Histochem Cytochem. 1987 Jan;35(1):33-7 PMID: 3794308
  6. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.
    J Histochem Cytochem. 1981 May;29(5):663-71 PMID: 6166664
  7. Structural determinants of Ricinus communis agglutinin and toxin specificity for oligosaccharides.
    J Biol Chem. 1979 Oct 10;254(19):9795-9 PMID: 489569
  8. Application of lectin--gold complexes for electron microscopic localization of glycoconjugates on thin sections.
    J Histochem Cytochem. 1983 Aug;31(8):987-99 PMID: 6190857
  9. Sizing of protein A-colloidal gold probes for immunoelectron microscopy.
    J Cell Biol. 1981 Aug;90(2):533-6 PMID: 7026575
  10. Coloidal gold, ferritin and peroxidase as markers for electron microscopic double labeling lectin techniques.
    J Histochem Cytochem. 1978 Mar;26(3):163-9 PMID: 632554
  11. Post-embedding localization of glycoconjugates by means of lectins on thin sections of tissues embedded in LR white.
    Histochem J. 1985 Dec;17(12):1321-36 PMID: 2422141
  12. A new method of preparing gold probes for multiple-labeling cytochemistry.
    Eur J Cell Biol. 1985 Jul;38(1):87-93 PMID: 4029177
  13. Applications of immunocolloids in light microscopy. III. Demonstration of antigenic and lectin-binding sites in semithin resin sections.
    J Histochem Cytochem. 1984 Oct;32(10):1075-83 PMID: 6384362
  14. Ultrastructural localization of lectin receptors on the luminal and abluminal aspects of brain micro-blood vessels.
    J Histochem Cytochem. 1986 Feb;34(2):251-61 PMID: 3753715
  15. The distribution of repeating [Gal beta 1,4GlcNAc beta 1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell lines BW5147 and PHAR 2.1.
    J Biol Chem. 1984 May 25;259(10):6253-60 PMID: 6725252
  16. Lectin affinity high-performance liquid chromatography. Interactions of N-glycanase-released oligosaccharides with Ricinus communis agglutinin I and Ricinus communis agglutinin II.
    J Biol Chem. 1987 Sep 5;262(25):12030-9 PMID: 3624246
  17. Demonstration of an extensive trans-tubular network continuous with the Golgi apparatus stack that may function in glycosylation.
    Cell. 1985 Nov;43(1):287-95 PMID: 3000603
  18. The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces.
    Biochim Biophys Acta. 1972 May 9;266(2):543-7 PMID: 4338881
  19. Carbohydrate binding studies on the lectin from Datura stramonium seeds.
    Arch Biochem Biophys. 1984 Jun;231(2):524-33 PMID: 6203486
  20. Prevention of non-specific interactions of gold-labeled reagents on tissue sections.
    Histochemistry. 1989;92(1):47-56 PMID: 2475468
  21. Lectin binding to parietal cells of human gastric mucosa.
    J Histochem Cytochem. 1986 Feb;34(2):237-43 PMID: 3511142
  22. Carbohydrate binding properties of complex-type oligosaccharides on immobilized Datura stramonium lectin.
    J Biol Chem. 1987 Feb 5;262(4):1602-7 PMID: 3805046
  23. Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins.
    Eur J Biochem. 1981 Jun;117(1):41-55 PMID: 7262089
  24. Light microscopic detection of sugar residues in glycoconjugates of salivary glands and the pancreas with lectin-horseradish peroxidase conjugates. I. Mouse.
    Histochem J. 1983 Dec;15(12):1217-38 PMID: 6643118
  25. Lectin affinity high-performance liquid chromatography: interactions of N-glycanase-released oligosaccharides with leukoagglutinating phytohemagglutinin, concanavalin A, Datura stramonium agglutinin, and Vicia villosa agglutinin.
    Anal Biochem. 1987 Nov 15;167(1):62-75 PMID: 3434801
Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00524763
Published
1989-00-00
Pages
515-22
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00524763
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2