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PMID: 2822394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP.

The EMBO journal ·Vol. 6 ·No. 8 ·1987-08-00 ·Pages 2449-56

Verner K, Schatz G

Abstract

We have studied the post-translational import of incomplete precursor chains into isolated yeast mitochondria. The precursor was a fusion protein containing a mitochondrial presequence attached to mouse dihydrofolate reductase. In vitro-synthesis of the precursor was interrupted by the elongation inhibitor cycloheximide and the arrested nascent chains cosedimenting with ribosomes were released by EDTA. These incomplete chains were efficiently imported by isolated yeast mitochondria; their import resembled that of the complete precursor in requiring an energized inner membrane and a mitochondrial presequence. It differed from that of the completed precursor in its resistance to methotrexate (which only binds to correctly folded dihydrofolate reductase) and its independence of added ATP. The incomplete chains were also more sensitive to proteinase K than the completed precursor. We conclude that the incomplete chains were incompletely folded and suggest that the lack of tight folding caused import into mitochondria to become independent of added ATP. This implies that ATP may participate, directly or indirectly, in the unfolding of the precursor for its transport into mitochondria.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Electron Transport Complex IV/genetics Intracellular Membranes/metabolism Mice Mitochondria/metabolism Protein Biosynthesis Protein Processing, Post-Translational Saccharomyces cerevisiae/genetics,metabolism Tetrahydrofolate Dehydrogenase/metabolism Transcription, Genetic
Chemicals
Adenosine Triphosphate Tetrahydrofolate Dehydrogenase Electron Transport Complex IV
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Verner K
Biocenter, University of Basel, Switzerland.
Schatz G
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34 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-08-00
Pages
2449-56
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553652
Subset
IM
Grants
NIGMS NIH HHS · CBY-1 1 R01 GM37803-01 · United States
External Links
PubMed source PMC Full Text View journal details
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