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PMID: 2823058 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro.

Molecular & general genetics : MGG ·Vol. 208 ·No. 3 ·1987-07-00 ·Pages 390-7

Lin LS, Kim YJ, Meyer RJ

Abstract

The broad host range plasmid R1162 contains a directly repeated, 20 bp DNA sequence in the region of the plasmid required in cis for replication and maintenance. This sequence has been chemically synthesized and cloned, and shown to be sufficient for expression of plasmid incompatibility. The sequence also inhibits replication of R1162 DNA in a cell-free system. The strengths of both these effects are determined by the number of direct repeats (DRs) present, and are also affected to similar degrees by different mutations within the repeated sequence. Several of the mutations were tested for their effect in cis on plasmid maintenance in the cell, and one was found to cause an increase in plasmid copy number. The results suggest that the direct repeats exert incompatibility by inhibiting DNA replication, presumably because they are the binding sites for a limiting essential protein.

MeSH Terms
Base Composition Base Sequence DNA Replication DNA Restriction Enzymes Escherichia coli/genetics Kinetics Oligodeoxyribonucleotides/chemical synthesis R Factors
Chemicals
Oligodeoxyribonucleotides DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lin L S
Department of Microbiology, University of Texas, Austin 78712-1095.
Kim Y J
Meyer R J
References (32)
32 references, click to expand
  1. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.
    J Bacteriol. 1976 Aug;127(2):982-7 PMID: 783124
  2. Vertical dye-buoyant density gradients for rapid analysis and preparation of plasmid DNA.
    Anal Biochem. 1981 Nov 15;118(1):191-3 PMID: 7337211
  3. Protein RepC is involved in copy number control of the broad host range plasmid RSF1010.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6090-4 PMID: 3898079
  4. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  5. P1 plasmid replication: multiple functions of RepA protein at the origin.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2588-92 PMID: 3857601
  6. Replication of prophage P1 during the cell cycle of Escherichia coli.
    Mol Gen Genet. 1977 Mar 28;152(1):71-6 PMID: 325389
  7. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  8. Copy number of the broad host-range plasmid R1162 is determined by the amounts of essential plasmid-encoded proteins.
    J Mol Biol. 1985 Oct 20;185(4):755-67 PMID: 2932556
  9. Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control.
    J Bacteriol. 1983 Jul;155(1):337-44 PMID: 6305917
  10. Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides.
    J Bacteriol. 1974 Nov;120(2):618-30 PMID: 4616941
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.
    Gene. 1981 Nov;15(2-3):257-71 PMID: 7028572
  13. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6 PMID: 333444
  14. Directly repeated, 20-bp sequence of plasmid R1162 DNA is required for replication, expression of incompatibility, and copy-number control.
    Plasmid. 1986 Jan;15(1):35-47 PMID: 3513218
  15. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  16. Plasmid P1 replication: negative control by repeated DNA sequences.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6456-60 PMID: 6387706
  17. The number of sex-factors per chromosome in Escherichia coli.
    Biochem J. 1971 Jan;121(1):93-103 PMID: 4940379
  18. Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.
    Nucleic Acids Res. 1982 Aug 11;10(15):4687-702 PMID: 6813825
  19. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  20. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  21. A simple and efficient method for chemical mutagenesis of DNA.
    Nucleic Acids Res. 1985 Mar 11;13(5):1733-45 PMID: 3889841
  22. Control of replication of the broad host range plasmid RSF1010: the incompatibility determinant consists of directly repeated DNA sequences.
    Mol Gen Genet. 1986 Apr;203(1):189-92 PMID: 3012279
  23. A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder.
    Anal Biochem. 1982 Apr;121(2):382-7 PMID: 6179438
  24. Replication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteins.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):654-8 PMID: 6322159
  25. Properties of R1162, a broad-host-range, high-copy-number plasmid.
    J Bacteriol. 1982 May;150(2):552-62 PMID: 6279561
  26. Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.
    J Mol Biol. 1975 Mar 15;92(4):529-40 PMID: 1097703
  27. Nucleotide sequence and functional properties of DNA encoding incompatibility in the broad host-range plasmid R1162.
    Mol Gen Genet. 1984;194(3):423-31 PMID: 6377013
  28. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  29. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.
    Anal Biochem. 1981 Apr;112(2):295-8 PMID: 6266279
  30. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  31. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  32. P1 plasmid replication: replicon structure.
    J Mol Biol. 1984 Mar 5;173(3):307-24 PMID: 6699914
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328129
Published
1987-07-00
Pages
390-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM36822 · United States
External Links
PubMed source DOI: 10.1007/BF00328129
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