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PMID: 6377013 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nucleotide sequence and functional properties of DNA encoding incompatibility in the broad host-range plasmid R1162.

Molecular & general genetics : MGG ·Vol. 194 ·No. 3 ·1984-00-00 ·Pages 423-31

Lin LS, Meyer RJ

Abstract

A 370 base pair (bp) fragment of R1162 DNA encoding the incompatibility determinant has been cloned and sequenced. The DNA is located between 6.1 and 6.5 on the R1162 map, near the origin of replication. The sequence contains three perfectly conserved 20 bp direct repeats, with 11 bp of this sequence repeated a fourth time. The direct repeat unit shows some homology with that of another, unrelated broad host-range plasmid, RK2. The cloned DNA has two other properties: it lowers the copy number of R1162 when cloned into this plasmid, and it is required in cis for replication of R1162 satellite plasmids.

MeSH Terms
Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics Plasmids Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lin L S
Meyer R J
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425554
Published
1984-00-00
Pages
423-31
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X00537
External Links
PubMed source DOI: 10.1007/BF00425554
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