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PMID: 2823071 Published · ppublish English Journal Article

RNA processing by RNase III is involved in the synthesis of Escherichia coli polynucleotide phosphorylase.

Molecular & general genetics : MGG ·Vol. 209 ·No. 1 ·1987-08-00 ·Pages 28-32

Takata R, Mukai T, Hori K

Abstract

The synthesis of Escherichia coli polynucleotide phosphorylase (PNPase) was examined in a mutant strain defective in the RNA processing enzyme RNase III (Rnc-). We found that the specific activity and the synthesis rate of PNPase were increased in the Rnc- strain by more than three times that in an Rnc+ strain. Such increased synthesis of PNPase was not observed in a mutant strain transformed with a plasmid carrying the rnc+ gene. Quantitative analysis of RNA showed that the transcripts from the pnp gene, which encodes PNPase, were degraded more slowly in the Rnc- strain than in the Rnc+ strain. These results indicate that processing of the transcripts by RNase III is intimately involved in controlling the expression of pnp by affecting the stability of its messenger RNA.

MeSH Terms
DNA Restriction Enzymes Endoribonucleases/metabolism Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes Genes, Bacterial Mutation Operon Polyribonucleotide Nucleotidyltransferase/biosynthesis,genetics RNA Processing, Post-Transcriptional Ribonuclease III Transcription, Genetic
Chemicals
Escherichia coli Proteins Polyribonucleotide Nucleotidyltransferase Endoribonucleases DNA Restriction Enzymes Ribonuclease III ribonuclease III, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takata R
Department of Biology, Saga Medical School, Japan.
Mukai T
Hori K
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329832
Published
1987-08-00
Pages
28-32
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00329832
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