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PMID: 2824195 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction of hybrid Tn501/Tn21 transposases in vivo: identification of a region of transposase conferring specificity of recognition of the 38-bp terminal inverted repeats.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2849-53

Evans LR, Brown NL

Abstract

In order to study the transposase enzymes of Class II prokaryotic transposable elements, we have constructed genes encoding hybrid transposase proteins. This was done by recombination in vivo between the tnpA genes of transposons Tn501 and Tn21. These hybrid genes can complement in trans a transposition-defective mutant of Tn501. The structures of the products of this complementation indicate whether the specificity of the hybrid transposase in recognising the 38 bp terminal inverted repeats is that of Tn501 or that of Tn21. The determinant of this specificity is in the N-terminal region of the transposase protein, between amino acids 28 and 216. The predicted amino acid sequences so far determined of transposases from the Class II family reveal an area of homology in this region.

MeSH Terms
Amino Acid Sequence Base Composition DNA Transposable Elements Escherichia coli/enzymology,genetics Genes Molecular Sequence Data Nucleic Acid Hybridization Nucleotide Mapping Nucleotidyltransferases/genetics Plasmids Repetitive Sequences, Nucleic Acid Transposases
Chemicals
DNA Transposable Elements Nucleotidyltransferases Transposases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Evans L R
Department of Biochemistry, University of Bristol, UK.
Brown N L
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25 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-09-00
Pages
2849-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553712
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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