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PMID: 2994007 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonas transposon Tn501.

Nucleic acids research ·Vol. 13 ·No. 15 ·1985-08-12 ·Pages 5657-69

Brown NL, Winnie JN, Fritzinger D, Pridmore RD

Abstract

The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr = 111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR gene. The amino acid sequence predicted from this reading frame shows 32% identity with that of the transposase of the related transposon Tn3. The C-terminal regions of these two polypeptides show slightly greater homology than the N-terminal regions when conservative amino acid substitutions are considered. With this sequence determination, the nucleotide sequence of Tn501 is fully defined. The main features of the sequence are briefly presented.

MeSH Terms
Amino Acid Sequence Base Sequence Codon DNA Transposable Elements DNA, Bacterial/genetics Genes, Bacterial Nucleotidyltransferases/genetics Pseudomonas aeruginosa/genetics Transcription, Genetic Transposases
Chemicals
Codon DNA Transposable Elements DNA, Bacterial Nucleotidyltransferases Transposases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brown N L
Winnie J N
Fritzinger D
Pridmore R D
References (36)
36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.15.5657
Published
1985-08-12
Pages
5657-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321896
Subset
IM
Databases
GENBANK
X02803
External Links
PubMed source DOI: 10.1093/nar/13.15.5657 PMC Full Text View journal details
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