Home LiteratureArticle Details
PMID: 6275390 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transposon-specified site-specific recombination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 1 ·1982-01-00 ·Pages 46-50

Kitts P, Symington L, Burke M, Reed R, Sherratt D

Abstract

Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product interchangeable with the Tn1/3tnpR protein in promoting Tn1/3 site-specific recombination. These data support the hypothesis that cointegrates containing directly repeated copies of Tn1/3 are obligatory intermediates in interreplicon transposition of Tn1/3. In addition, we show here that the reaction is independent of the element-encoded tnpA gene product. Tn501, which specifies mercury resistance, also produces cointegrates as intermediates in interreplicon transposition. The appearance of Tn501-specified recombination activity that can act on these cointegrates requires growth of cells in the presence of Hg2+.

MeSH Terms
DNA Transposable Elements DNA, Recombinant Escherichia coli/genetics Genetic Complementation Test Plasmids
Chemicals
DNA Transposable Elements DNA, Recombinant
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kitts P
Symington L
Burke M
Reed R
Sherratt D
References (21)
21 references, click to expand
  1. Mercury resistance in a plasmid-bearing strain of Escherichia coli.
    J Bacteriol. 1972 Dec;112(3):1228-36 PMID: 4565536
  2. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3.
    J Bacteriol. 1978 Nov;136(2):742-56 PMID: 361721
  3. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6012-6 PMID: 366607
  4. The control region of the F sex factor DNA transfer cistrons: restriction mapping and DNA cloning.
    Mol Gen Genet. 1978 Oct 24;165(3):295-304 PMID: 368564
  5. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1.
    Nature. 1979 Feb 8;277(5696):453-6 PMID: 368646
  6. The gamma delta sequence of F is an insertion sequence.
    J Mol Biol. 1978 Dec 15;126(3):347-65 PMID: 745233
  7. Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element.
    Mol Gen Genet. 1979 Mar 9;171(1):7-13 PMID: 375023
  8. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1933-7 PMID: 287033
  9. Chromosome transfer and Hfr formation by F in rec+ and recA strains of Escherichia coli K12.
    Plasmid. 1979 Jul;2(3):358-65 PMID: 384417
  10. Identification and characterization of a self-regulated repressor of translocation of the Tn3 element.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):4020-4 PMID: 386354
  11. Transposition of the Escherichia coli insertion element gamma generates a five-base-pair repeat.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4882-6 PMID: 388421
  12. Identification of the protein encoded by the transposable element Tn3 which is required for its transposition.
    Nature. 1979 Dec 20-27;282(5741):797-801 PMID: 390401
  13. Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product.
    Nature. 1979 Dec 20-27;282(5741):801-6 PMID: 390402
  14. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3.
    Cell. 1979 Dec;18(4):1153-63 PMID: 391406
  15. Dissection of the transposition process: a transposon-encoded site-specific recombination system.
    Mol Gen Genet. 1979 Oct 1;175(3):267-74 PMID: 392228
  16. Nucleotide sequences at the ends of the mercury resistance transposon, Tn501.
    Nucleic Acids Res. 1980 May 10;8(9):1933-45 PMID: 6253951
  17. Analysis of the nucleotide sequence of an invertible controlling element.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4196-200 PMID: 6933466
  18. Resolution of cointegrates between transposons gamma delta and Tn3 defines the recombination site.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3428-32 PMID: 6267590
  19. Analysis of the structure and function of the kanamycin-resistance transposon Tn903.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:125-33 PMID: 6271455
  20. Identification of a sex-factor-affinity site in E. coli as gamma delta.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:135-40 PMID: 6271456
  21. Transposon-specified, site-specific recombination systems.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:275-81 PMID: 6271476
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.1.46
Published
1982-01-00
Pages
46-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345658
Subset
IM
Grants
NIGMS NIH HHS · GM 26154-10 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.1.46 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2