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PMID: 368564 Published · ppublish English Journal Article

The control region of the F sex factor DNA transfer cistrons: restriction mapping and DNA cloning.

Molecular & general genetics : MGG ·Vol. 165 ·No. 3 ·1978-10-24 ·Pages 295-304

Thompson R, Achtman M

Abstract

A restriction endonuclease map of EcoRI fragment f6 of F sex factor DNA was constructed and aligned with pre-existing physical and genetic maps. Results of genetic complementation tests and analysis of proteins synthesized in minicells from PstI and BglII sub-fragment clones, or from a specific BglII fragment deletion, have allowed mapping of the locations of the origin of DNA transfer and many of the transfer genes known to lie on f6. The proteins detected account for 78% of the coding capacity of fragment f6.

MeSH Terms
Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Bacterial/genetics Escherichia coli/genetics F Factor Genes Genetic Complementation Test Genetic Linkage Operon
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thompson R
Achtman M
References (23)
23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332530
Published
1978-10-24
Pages
295-304
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332530
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