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PMID: 2825174 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of temperature-sensitive Abelson virus mutants by site-directed mutagenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 22 ·1987-11-00 ·Pages 8021-5

Engelman A, Rosenberg N

Abstract

Mutants of Abelson virus encoding temperature-sensitive protein-tyrosine kinase (EC 2.7.1.112) were created by site-directed mutagenesis using sequence information from temperature-sensitive mutants of the related v-src oncogene. Expression of these two independent mutations in Escherichia coli resulted in reduced phosphorylation of the mutant proteins at high temperature. Viruses containing one of the mutations induced conditional transformation of both NIH 3T3 and lymphoid cells when expressed in the context of a truncated transforming protein. These results underscore the functional homology between protein-tyrosine kinases and suggest that transfer of mutations within a related gene family may provide a rapid method to create mutants.

MeSH Terms
Abelson murine leukemia virus/genetics Amino Acid Sequence Animals Cell Line Cell Transformation, Viral Fibroblasts Leukemia Virus, Murine/genetics Mice Molecular Sequence Data Mutation Phosphorylation Protein-Tyrosine Kinases/genetics,metabolism Retroviridae Proteins/genetics,metabolism Sequence Homology, Nucleic Acid Temperature Viral Proteins/genetics,metabolism
Chemicals
Retroviridae Proteins Viral Proteins Protein-Tyrosine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Engelman A
Department of Molecular Biology, Tufts University School of Medicine, Boston, MA 02111.
Rosenberg N
References (47)
47 references, click to expand
  1. Temperature sensitive mutants of an avian sarcoma virus.
    Virology. 1969 Dec;39(4):930-1 PMID: 4311641
  2. ret transforming gene encodes a fusion protein homologous to tyrosine kinases.
    Mol Cell Biol. 1987 Apr;7(4):1378-85 PMID: 3037315
  3. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant.
    Virology. 1971 Nov;46(2):470-9 PMID: 4331732
  4. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail.
    Cell. 1977 May;11(1):95-103 PMID: 194709
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. Identification of an Abelson murine leukemia virus-encoded protein present in transformed fibroblast and lymphoid cells.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2488-92 PMID: 209468
  7. Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3974-8 PMID: 211510
  8. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  9. The transforming gene of Moloney murine sarcoma virus.
    Nature. 1979 Nov 22;282(5737):378-83 PMID: 503218
  10. Abelson murine leukemia virus transformation-defective mutants with impaired P120-associated protein kinase activity.
    J Virol. 1980 Nov;36(2):374-86 PMID: 6253663
  11. Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3932-6 PMID: 6449003
  12. A transformation-defective mutant of Abelson murine leukemia virus lacks protein kinase activity.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4993-7 PMID: 6254050
  13. A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation.
    Cell. 1980 Dec;22(3):767-75 PMID: 6257397
  14. Structure of the Abelson murine leukemia virus genome and the homologous cellular gene: studies with cloned viral DNA.
    Cell. 1980 Dec;22(3):777-85 PMID: 6257398
  15. Abelson murine leukemia virus mutants deficient in kinase activity and lymphoid cell transformation.
    J Virol. 1980 Dec;36(3):766-74 PMID: 6257926
  16. Transposon-mediated site-specific recombination: a defined in vitro system.
    Cell. 1981 Sep;25(3):713-9 PMID: 6269755
  17. Mapping of nonconditional and conditional mutants in the src gene of Prague strain Rous sarcoma virus.
    Virology. 1982 Jan 15;116(1):72-83 PMID: 6278718
  18. Antitermination and termination functions of the cloned nutL, N, and tL1 modules of coliphage lambda.
    Gene. 1981 Dec;16(1-3):261-74 PMID: 6211393
  19. Transfection of fibroblasts by cloned Abelson murine leukemia virus DNA and recovery of transmissible virus by recombination with helper virus.
    J Virol. 1982 Jan;41(1):271-85 PMID: 6283119
  20. Hormone-dependent terminal differentiation in vitro of chicken erythroleukemia cells transformed by ts mutants of avian erythroblastosis virus.
    Cell. 1982 Apr;28(4):907-19 PMID: 6954007
  21. Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses.
    Cell. 1982 Oct;30(3):787-95 PMID: 6291784
  22. Expression of an Abelson murine leukemia virus-encoded protein in Escherichia coli causes extensive phosphorylation of tyrosine residues.
    J Biol Chem. 1982 Nov 25;257(22):13181-4 PMID: 6292177
  23. Isolation and characterization of a temperature-sensitive mutant of avian myeloblastosis virus.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1421-5 PMID: 6298790
  24. Site-directed deletions of Abelson murine leukemia virus define 3' sequences essential for transformation and lethality.
    J Virol. 1983 Mar;45(3):1028-36 PMID: 6300444
  25. Cloning and analysis of reverse transcript P160 genomes of Abelson murine leukemia virus.
    J Virol. 1983 Mar;45(3):1195-9 PMID: 6300457
  26. Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3623-7 PMID: 6304726
  27. Cellular oncogenes and retroviruses.
    Annu Rev Biochem. 1983;52:301-54 PMID: 6351725
  28. The erbB gene of avian erythroblastosis virus is a member of the src gene family.
    Cell. 1983 Nov;35(1):71-8 PMID: 6313229
  29. Rous sarcoma virus contains sequences which permit expression of the gag gene in Escherichia coli.
    Mol Cell Biol. 1983 Oct;3(10):1746-58 PMID: 6316124
  30. Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.
    DNA. 1984 Dec;3(6):479-88 PMID: 6096101
  31. Molecular basis of host range variation in avian retroviruses.
    J Virol. 1985 Jan;53(1):32-9 PMID: 2981357
  32. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  33. The minimum transforming region of v-abl is the segment encoding protein-tyrosine kinase.
    J Virol. 1985 Apr;54(1):114-22 PMID: 2983108
  34. Two independent mutations are required for temperature-sensitive cell transformation by a Rous sarcoma virus temperature-sensitive mutant.
    J Virol. 1985 Dec;56(3):743-9 PMID: 2999430
  35. The human met oncogene is related to the tyrosine kinase oncogenes.
    Nature. 1985 Nov 28-Dec 4;318(6044):385-8 PMID: 4069211
  36. A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA.
    Cell. 1985 Dec;43(2 Pt 1):393-404 PMID: 2416464
  37. The neu oncogene encodes an epidermal growth factor receptor-related protein.
    Nature. 1986 Jan 16-22;319(6050):226-30 PMID: 3945311
  38. A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences.
    Nature. 1986 Feb 27-Mar 5;319(6056):743-8 PMID: 2869410
  39. A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family.
    Nature. 1986 Apr 3-9;320(6061):415-21 PMID: 3007997
  40. Localization of temperature-sensitive transformation mutations and back mutations in the Rous sarcoma virus src gene.
    J Virol. 1986 May;58(2):694-9 PMID: 3009882
  41. yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5459-63 PMID: 3526330
  42. Gag-derived but not abl-derived determinants are exposed on the surface of Abelson virus-transformed cells.
    Virology. 1986 Oct 30;154(2):286-301 PMID: 3020782
  43. Isolation and oncogenic potential of a novel human src-like gene.
    Mol Cell Biol. 1986 Dec;6(12):4195-201 PMID: 3099169
  44. Isolation of temperature-sensitive tyrosine kinase mutants of v-abl oncogene by screening with antibodies for phosphotyrosine.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1345-9 PMID: 2434953
  45. Sevenless, a cell-specific homeotic gene of Drosophila, encodes a putative transmembrane receptor with a tyrosine kinase domain.
    Science. 1987 Apr 3;236(4797):55-63 PMID: 2882603
  46. A temperature-sensitive mutant of Abelson murine leukemia virus confers inducibility of IgM expression to transformed lymphoid cells.
    EMBO J. 1987 Apr;6(4):951-6 PMID: 3036494
  47. Rous sarcoma virus: a function required for the maintenance of the transformed state.
    Nature. 1970 Sep 5;227(5262):1021-3 PMID: 4317808
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.22.8021
Published
1987-11-00
Pages
8021-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299468
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.22.8021 PMC Full Text View journal details
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