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PMID: 2825183 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8267-71

Dean FB, Borowiec JA, Ishimi Y, Deb S, Tegtmeyer P, Hurwitz J

Abstract

Simian virus 40 (SV40) large tumor antigen (T antigen) unwinds DNA containing the SV40 origin of replication. The origin requirement for unwinding can be satisfied by the 64-base-pair SV40 core origin that supports T-antigen-dependent DNA replication both in vivo and in vitro. The core origin contains three domains with specific DNA sequence features. These include an inverted repeat, a central T-antigen binding domain, and an adenine- and thymine-rich domain containing a DNA bending focus. The domain and spacer requirements of the core origin for DNA unwinding and replication in vitro are strikingly similar to the origin requirements for DNA replication in vivo. Thus, each of the three functional domains of the core origin contributes directly to the initiation of duplex DNA unwinding by T antigen.

MeSH Terms
DNA Helicases DNA Mutational Analysis DNA Replication DNA, Viral/genetics DNA-Binding Proteins Simian virus 40/genetics Virus Replication
Chemicals
DNA, Viral DNA-Binding Proteins DNA Helicases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dean F B
Graduate Program in Molecular Biology, Sloan Kettering Cancer Center, New York, NY 10021.
Borowiec J A
Ishimi Y
Deb S
Tegtmeyer P
Hurwitz J
References (27)
27 references, click to expand
  1. The binding site on SV40 DNA for a T antigen-related protein.
    Cell. 1978 Jan;13(1):165-79 PMID: 202398
  2. Initiation of simian virus 40 DNA replication in vitro: large-tumor-antigen- and origin-dependent unwinding of the template.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3643-7 PMID: 3035543
  3. Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.
    J Virol. 1983 Apr;46(1):143-50 PMID: 6298451
  4. Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
    J Virol. 1983 Apr;46(1):151-61 PMID: 6298452
  5. Simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6973-7 PMID: 6095264
  6. Monomers through trimers of large tumor antigen bind in region I and monomers through tetramers bind in region II of simian virus 40 origin of replication DNA as stable structures in solution.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3626-30 PMID: 2987937
  7. In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5710-4 PMID: 2994044
  8. T antigen and template requirements for SV40 DNA replication in vitro.
    EMBO J. 1985 Nov;4(11):2933-9 PMID: 2998767
  9. Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.
    J Virol. 1986 Jan;57(1):138-44 PMID: 3001340
  10. An altered DNA conformation in origin region I is a determinant for the binding of SV40 large T antigen.
    Cell. 1986 Mar 14;44(5):719-25 PMID: 3004744
  11. Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome.
    Cell. 1986 Apr 11;45(1):53-64 PMID: 3006926
  12. The Escherichia coli dnaB replication protein is a DNA helicase.
    J Biol Chem. 1986 Apr 5;261(10):4738-48 PMID: 3007474
  13. Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2869-73 PMID: 3010320
  14. Simian virus 40 DNA replication in vitro: study of events preceding elongation of chains.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4612-6 PMID: 2425351
  15. Multiple DNA-protein interactions governing high-precision DNA transactions.
    Science. 1986 Sep 5;233(4768):1050-6 PMID: 2943018
  16. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
    Mol Cell Biol. 1985 Aug;5(8):2051-60 PMID: 3018548
  17. DNA helicase activity of SV40 large tumor antigen.
    EMBO J. 1986 Aug;5(8):1939-44 PMID: 3019672
  18. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7638-42 PMID: 3020552
  19. Functional organization of the simian virus 40 origin of DNA replication.
    Mol Cell Biol. 1986 Apr;6(4):1117-28 PMID: 3023870
  20. Domain structure of the simian virus 40 core origin of replication.
    Mol Cell Biol. 1986 May;6(5):1663-70 PMID: 3023900
  21. The adenine-thymine domain of the simian virus 40 core origin directs DNA bending and coordinately regulates DNA replication.
    Mol Cell Biol. 1986 Dec;6(12):4578-84 PMID: 3025662
  22. Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):16-20 PMID: 3025851
  23. Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication.
    J Virol. 1987 Feb;61(2):411-8 PMID: 3027371
  24. Roles of DNA topoisomerases in simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1987 Feb;84(4):950-4 PMID: 3029765
  25. Replication of simian virus 40 origin-containing DNA in vitro with purified proteins.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1834-8 PMID: 3031654
  26. The T-antigen-binding domain of the simian virus 40 core origin of replication.
    J Virol. 1987 Jul;61(7):2143-9 PMID: 3035215
  27. Territorial limits and functional anatomy of the simian virus 40 replication origin.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):381-5 PMID: 6281769
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8267
Published
1987-12-00
Pages
8267-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299523
Subset
IM
Grants
NIGMS NIH HHS · 5RO1-GM34559 · United States
NCI NIH HHS · R01-CA18808 · United States
NCI NIH HHS · R01-CA38146 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.23.8267 PMC Full Text View journal details
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