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PMID: 3027371 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication.

Journal of virology ·Vol. 61 ·No. 2 ·1987-02-00 ·Pages 411-8

Wiekowski M, Dröge P, Stahl H

Abstract

Various monoclonal antibodies specific for simian virus 40 large tumor antigen (T antigen) inhibit the elongation process of viral DNA replication in an in vitro system. The results provide strong evidence for a function intrinsic to T antigen during ongoing replicative-chain elongation. The antibody inhibition studies were further used to establish a correlation between the known biochemical activities of T antigen and its function during the elongation phase. The data demonstrate that, in addition to DNA binding and ATPase, a third function of T antigen is required for replicative chain elongation. This function is most probably related to the recently described DNA helicase activity of T antigen. This conclusion is based on the following results: aphidicolin treatment of actively replicating simian virus 40 minichromosomes causes a partial uncoupling of parental DNA strand separation and DNA synthesis; the strand separation reaction is blocked by the same monoclonal antibodies which strongly inhibit the elongation process. DNA helicase activity of isolated T antigen is equally well inhibited by the same set of monoclonal antibodies that affect minichromosome replication in vitro.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex/analysis Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/metabolism Cell Line Chromatin/metabolism DNA Replication Kinetics Mice Oncogene Proteins, Viral/metabolism Protein Kinases/metabolism Simian virus 40/enzymology,genetics
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Chromatin Oncogene Proteins, Viral Protein Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wiekowski M
Dröge P
Stahl H
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45 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.2.411-418.1987
Published
1987-02-00
Pages
411-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253964
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.2.411-418.1987 PMC Full Text View journal details
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