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PMID: 6310151 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

In vitro DNA synthesis by an alpha-like DNA polymerase bound to replicating simian virus 40 chromosomes.

Journal of virology ·Vol. 48 ·No. 1 ·1983-10-00 ·Pages 304-8

Waqar MA, Evans MJ, Burke JF, Tsubota Y, Plummer MJ, Huberman JA

Abstract

Simian virus 40 chromosomes carry out replicative DNA synthesis in vitro which is sensitive to aphidicolin and to N-ethylmaleimide, resistant to 2',3'-dideoxythymidine-5'-triphosphate, and proportional to the amount of chromosome-associated alpha-like polymerase. Thus, an alpha-like DNA polymerase (alpha polymerase or delta polymerase) is responsible for in vitro DNA synthesis.

MeSH Terms
Aphidicolin DNA Polymerase II/metabolism DNA Replication DNA, Viral/biosynthesis DNA-Directed DNA Polymerase/metabolism Dideoxynucleotides Diterpenes/pharmacology Ethylmaleimide/pharmacology Simian virus 40/enzymology Thymine Nucleotides/pharmacology
Chemicals
DNA, Viral Dideoxynucleotides Diterpenes Thymine Nucleotides Aphidicolin DNA Polymerase II DNA-Directed DNA Polymerase 2',3'-dideoxythymidine triphosphate Ethylmaleimide
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Waqar M A
Evans M J
Burke J F
Tsubota Y
Plummer M J
Huberman J A
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.48.1.304-308.1983
Published
1983-10-00
Pages
304-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255347
Subset
IM
Grants
NCI NIH HHS · CA-22655 · United States
External Links
PubMed source DOI: 10.1128/JVI.48.1.304-308.1983 PMC Full Text View journal details
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