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PMID: 6264157 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Polyoma virus minichromosomes: associated enzyme activities.

Journal of virology ·Vol. 38 ·No. 3 ·1981-06-00 ·Pages 826-32

Gourlie BB, Pigiet V, Breaux CB, Krauss MR, King CR, Benbow RM

Abstract

Polyoma minichromosomes were isolated and fractionated on glycerol gradients as described by Gourlie et al. (J. Virol. 38:805-814, 1981). Specific assays for DNa polymerases alpha, beta, and gamma, DNA topoisomerase I, and RNase H were carried out on each fraction. The number of units of activity in each fraction was compared with the number of total polyoma and replicative intermediate DNA molecules in each fraction determined by quantitative electron microscopy (M. R. Krauss and R. M. Benbow, J. Virol. 38:815-825, 1981). DNA polymerase alpha cosedimented with polyoma replicative intermediate DNA molecules. DNA polymerase beta and DNA topoisomerase I activities sedimented with mature polyoma minichromosomes. Although the bulk of RNase H activity sedimented in the minichromosome region, the peak of activity was found one fraction behind the peak of mature minichromosomes. Virtually no DNA polymerase gamma activity cosedimented with polyoma minichromosomes.

MeSH Terms
Centrifugation, Density Gradient DNA Topoisomerases, Type I/metabolism DNA, Viral/biosynthesis DNA-Directed DNA Polymerase/metabolism Endonucleases/metabolism Genes, Viral Polyomavirus/enzymology,genetics Replicon Ribonuclease H Ribonucleases/metabolism
Chemicals
DNA, Viral DNA-Directed DNA Polymerase Endonucleases Ribonucleases Ribonuclease H DNA Topoisomerases, Type I
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gourlie B B
Pigiet V
Breaux C B
Krauss M R
King C R
Benbow R M
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35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.3.826-832.1981
Published
1981-06-00
Pages
826-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171219
Subset
IM
Grants
NCI NIH HHS · CA06304 · United States
NIGMS NIH HHS · GM-23813 · United States
NIGMS NIH HHS · GM-23970 · United States
External Links
PubMed source DOI: 10.1128/JVI.38.3.826-832.1981 PMC Full Text View journal details
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