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PMID: 2825188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Escherichia coli replication factor Y, a component of the primosome, can act as a DNA helicase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8345-9

Lee MS, Marians KJ

Abstract

The primosome is a mobile multienzyme DNA replication-priming complex that requires seven Escherichia coli proteins for assembly (the products of the dnaB, dnaC, dnaG, and dnaT genes as well as proteins n and n" and replication factor Y). It has been shown previously that the primosome, in combination with the E. coli DNA polymerase III holoenzyme, can form replication forks in vitro that move at rates similar to those measured in vivo and that the primosome and one of the components of the primosome, the DNA B protein, have DNA helicase activity. Evidence is presented here that another component of the primosome, replication factor Y, possesses DNA helicase activity as well. Factor Y helicase activity requires the presence of E. coli single-stranded DNA binding protein, Mg2+, and hydrolyzable ATP or dATP. Helicase activity is stimulated 15-fold when the enzyme is actively loaded onto single-stranded DNA through a primosome assembly site, and duplex DNA is unwound unidirectionally, 3'----5', along the DNA strand to which the protein is bound.

MeSH Terms
Adenosine Triphosphatases/physiology Binding Sites DNA Helicases/genetics DNA Replication DNA, Single-Stranded/genetics DNA-Binding Proteins/physiology DnaB Helicases Multienzyme Complexes/physiology Regulatory Sequences, Nucleic Acid
Chemicals
DNA, Single-Stranded DNA-Binding Proteins Multienzyme Complexes Adenosine Triphosphatases dnaB protein, E coli DNA Helicases DnaB Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee M S
Graduate Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Marians K J
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8345
Published
1987-12-00
Pages
8345-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299539
Subset
IM
Grants
NIGMS NIH HHS · GM 34557 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.23.8345 PMC Full Text View journal details
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