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PMID: 6273849 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Escherichia coli factor Y sites of plasmid pBR322 can function as origins of DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 10 ·1981-10-00 ·Pages 6111-5

Zipursky SL, Marians KJ

Abstract

The Escherichia coli replication factor Y, in conjunction with other genetically undefined E. coli replication factors and the gene products of the E. coli dnaB, dnaC, and dnaG loci, is involved in de novo primer formation on the phi X174 (+) single-strand circular DNA template [(+) ss(c)DNA]. The participation of factor Y in this series of reactions is correlated with its phi X174 (+) ss(c)-specific DNA-dependent ATPase activity. Recently two factor Y effector DNA segments of the plasmid pBR322 have been identified in close proximity to the plasmid origin of DNA replication. We report here that insertion of these factor Y sites into the filamentous phage f1R229 (+) ss(c)DNA confers upon it the ability to be converted to RF DNA in vitro through a rifampicin-resistant dnaB, dnaC, and dnaG gene product-dependent pathway. Our data suggest that factor Y effector sites can function as origins of DNA replication.

MeSH Terms
Bacteriophage phi X 174/genetics DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics DNA, Recombinant/metabolism DNA, Viral/genetics Escherichia coli/genetics Genotype Mutation Plasmids Templates, Genetic
Chemicals
DNA, Bacterial DNA, Recombinant DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zipursky S L
Marians K J
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.10.6111
Published
1981-10-00
Pages
6111-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348987
Subset
IM
Grants
NIGMS NIH HHS · 2 R01 GM13344 · United States
NIGMS NIH HHS · 5 R01 GM26410 · United States
NIGMS NIH HHS · GM07491 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.10.6111 PMC Full Text View journal details
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