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PMID: 7005899 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6566-70

Nomura N, Ray DS

Abstract

In order to investigate initiation of H-strand (lagging strand) replication of the plasmid ColE1, the origin region fragment (Hae II-E) of ColE1 was inserted into the intergenic region of filamentous DNA phage M13 and cloned. A site capable of promoting DNA strand initiation on a single-stranded DNA template has been detected on the L-strand (leading strand) of the cloned fragment. The site, named rri-1 rifampicin-resistant initiation), directs conversion of chimeric phage single-stranded DNA to parental replicative form in the presence of rifampicin, which blocks the function of the complementary strand origin of M13. The function of rri-1 is dependent on both the dnaG and dnaB gene products. It is postulated that rri-1 might be an initiation site for synthesis of the lagging DNA strand during unidirectional replication of ColE1 DNA.

MeSH Terms
Bacterial Proteins/metabolism Bacteriocin Plasmids Base Sequence Coliphages/genetics DNA Replication/drug effects DNA, Single-Stranded/genetics DNA, Viral/genetics Escherichia coli/genetics Plasmids Rifampin/pharmacology
Chemicals
Bacterial Proteins DNA, Single-Stranded DNA, Viral Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nomura N
Ray D S
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6566
Published
1980-11-00
Pages
6566-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350326
Subset
IM
Grants
NIAID NIH HHS · AI 10752-08 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.11.6566 PMC Full Text View journal details
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