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PMID: 2825419 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Primary structure of the glycoprotein E2 of coronavirus MHV-A59 and identification of the trypsin cleavage site.

Virology ·Vol. 161 ·No. 2 ·1987-12-00 ·Pages 479-87

Luytjes W, Sturman LS, Bredenbeek PJ, Charite J, van der Zeijst BA, Horzinek MC, Spaan WJ

Abstract

The nucleotide sequence of the peplomer (E2) gene of MHV-A59 was determined from a set of overlapping cDNA clones. The E2 gene encodes a protein of 1324 amino acids including a hydrophobic signal peptide. A second large hydrophobic domain is found near the COOH terminus and probably represents the membrane anchor. Twenty glycosylation sites are predicted. Cleavage of the E2 protein results in two different 90K species, 90A and 90B (L.S. Sturman, C. S. Ricard, and K. V. Holmes (1985) J. Virol. 56, 904-911), and activates cell fusion. Protein sequencing of the trypsin-generated N-terminus revealed the position of the cleavage site. 90A and 90B could be identified as the C-terminal and the N-terminal parts, respectively. Amino acid sequence comparison of the A59 and JHM E2 proteins showed extensive homology and revealed a stretch of 89 amino acids in the 90B region of the A59 E2 protein that is absent in JHM.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics Genes, Viral Glycoproteins/genetics Molecular Sequence Data Murine hepatitis virus/genetics Trypsin/metabolism Viral Proteins/genetics
Chemicals
Glycoproteins Viral Proteins DNA Trypsin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Luytjes W
Institute of Virology, Veterinary Faculty, State University of Utrecht, The Netherlands.
Sturman L S
Bredenbeek P J
Charite J
van der Zeijst B A
Horzinek M C
Spaan W J
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1016/0042-6822(87)90142-5
Published
1987-12-00
Pages
479-87
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7130946
Subset
IM
Grants
NIGMS NIH HHS · GM 31698 · United States
Databases
GENBANK
M18379
External Links
PubMed source DOI: 10.1016/0042-6822(87)90142-5 PMC Full Text View journal details
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