Home LiteratureArticle Details
PMID: 2826129 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Xfin: an embryonic gene encoding a multifingered protein in Xenopus.

The EMBO journal ·Vol. 6 ·No. 10 ·1987-10-00 ·Pages 3065-70

Ruiz i Altaba A, Perry-O'Keefe H, Melton DA

Abstract

The Xenopus laevis genome was screened for putative DNA-binding gene products by using the 'finger' region of the Drosophila gene Krüppel as a probe. The one gene detected, named Xfin, codes for a protein with 37 finger domains that comprise nearly 90% of the protein. In the light of studies by Rhodes and Klug (Cell, 46, 123-132, 1986), these data suggest that the Xfin protein has the capacity to bind an unusually large stretch (185 bases) of DNA. The Xfin gene is expressed as a maternal and zygotic mRNA that undergoes extensive polyadenylation changes during early development. The Xfin mRNA expression pattern and the potential DNA binding activity of the protein point to the possibility that the Xfin gene may have a role in controlling gene activity during early embryonic development.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA/analysis DNA Restriction Enzymes DNA-Binding Proteins/genetics Embryo, Nonmammalian/physiology Genes Molecular Sequence Data RNA, Messenger/genetics Transcription, Genetic Xenopus laevis/embryology
Chemicals
DNA-Binding Proteins RNA, Messenger DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ruiz i Altaba A
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Perry-O'Keefe H
Melton D A
References (41)
41 references, click to expand
  1. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
    J Morphol. 1972 Feb;136(2):153-79 PMID: 4109871
  2. C-myc expression is dissociated from DNA synthesis and cell division in Xenopus oocyte and early embryonic development.
    EMBO J. 1986 Dec 20;5(13):3571-7 PMID: 3549281
  3. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  4. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  5. A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
    Cell. 1982 Oct;30(3):675-86 PMID: 6183003
  6. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  7. Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes.
    J Mol Biol. 1983 May 25;166(3):309-27 PMID: 6854649
  8. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  9. Differential gene expression in the gastrula of Xenopus laevis.
    Science. 1983 Oct 14;222(4620):135-9 PMID: 6688681
  10. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  11. Transcription of class III genes: formation of preinitiation complexes.
    Science. 1983 Nov 18;222(4625):740-8 PMID: 6356356
  12. Cloning of an X. laevis gene expressed during early embryogenesis coding for a peptide region homologous to Drosophila homeotic genes.
    Cell. 1984 Jun;37(2):409-14 PMID: 6327066
  13. Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
    Cell. 1984 Jun;37(2):645-52 PMID: 6722885
  14. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  15. A homeo-box-containing gene expressed during oogenesis in Xenopus.
    Cell. 1984 Nov;39(1):157-62 PMID: 6207937
  16. Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
    Cell. 1984 Dec;39(3 Pt 2):479-89 PMID: 6210149
  17. Two divergent cellular src genes are expressed in Xenopus laevis.
    Nucleic Acids Res. 1985 Mar 11;13(5):1747-61 PMID: 2987836
  18. The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
    FEBS Lett. 1985 Jul 8;186(2):271-4 PMID: 4007166
  19. Deletion analysis identifies a region, upstream of the ADH2 gene of Saccharomyces cerevisiae, which is required for ADR1-mediated derepression.
    Mol Cell Biol. 1985 Jul;5(7):1743-9 PMID: 3160930
  20. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  21. Identification and cloning of localized maternal RNAs from Xenopus eggs.
    Cell. 1985 Oct;42(3):769-77 PMID: 2414011
  22. Sequence and structure of the serendipity locus of Drosophila melanogaster. A densely transcribed region including a blastoderm-specific gene.
    J Mol Biol. 1985 Nov 5;186(1):149-66 PMID: 3935797
  23. Steroid receptor regulated transcription of specific genes and gene networks.
    Annu Rev Genet. 1985;19:209-52 PMID: 3909942
  24. Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.
    EMBO J. 1985 Dec 16;4(13A):3463-71 PMID: 4092685
  25. Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA.
    Nature. 1986 Mar 20-26;320(6059):283-7 PMID: 3515197
  26. Structure of the gene for Xenopus transcription factor TFIIIA.
    Nucleic Acids Res. 1986 Mar 11;14(5):2187-200 PMID: 3754326
  27. Potential metal-binding domains in nucleic acid binding proteins.
    Science. 1986 Apr 25;232(4749):485-7 PMID: 2421409
  28. An underlying repeat in some transcriptional control sequences corresponding to half a double helical turn of DNA.
    Cell. 1986 Jul 4;46(1):123-32 PMID: 3013416
  29. Embryonic expression and nuclear localization of Xenopus homeobox (Xhox) gene products.
    EMBO J. 1986 Jun;5(6):1237-44 PMID: 3015593
  30. Gene regulation. Fingers and DNA half-turns.
    Nature. 1986 Aug 14-20;322(6080):597-8 PMID: 3748138
  31. Bifunctional messenger RNAs in eukaryotes.
    Cell. 1986 Nov 21;47(4):481-3 PMID: 3779834
  32. Two forms of the Ia antigen-associated invariant chain result from alternative initiations at two in-phase AUGs.
    Cell. 1986 Nov 21;47(4):619-25 PMID: 3779839
  33. A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene.
    Cell. 1986 Dec 26;47(6):1025-32 PMID: 3096579
  34. Fingers and helices.
    Nature. 1986 Nov 20-26;324(6094):215 PMID: 3785392
  35. Expression of the c-myc proto-oncogene during development of Xenopus laevis.
    Mol Cell Biol. 1986 Dec;6(12):4499-508 PMID: 3540613
  36. The c-erb-A protein is a high-affinity receptor for thyroid hormone.
    Nature. 1986 Dec 18-31;324(6098):635-40 PMID: 2879242
  37. The c-erb-A gene encodes a thyroid hormone receptor.
    Nature. 1986 Dec 18-31;324(6098):641-6 PMID: 2879243
  38. EXAFS study of the zinc-binding sites in the protein transcription factor IIIA.
    Nature. 1986 Dec 18-31;324(6098):698-9 PMID: 3796733
  39. Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor.
    Nature. 1987 Jan 1-7;325(6099):75-8 PMID: 3025750
  40. Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.
    EMBO J. 1986 Dec 20;5(13):3563-70 PMID: 3549280
  41. Methods for nuclear transplantation in amphibia.
    Methods Cell Biol. 1977;16:125-39 PMID: 329056
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-10-00
Pages
3065-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553744
Subset
IM
Databases
GENBANK
X06021
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2