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PMID: 282636 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Idiotypic analysis of antibodies to poly(Glu60Ala30Tyr10): interstrain and interspecies idiotypic crossreactions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 12 ·1978-12-00 ·Pages 6192-6

Ju ST, Benacerraf B, Dorf ME

Abstract

A common idiotype was defined by a guinea pig anti-idiotypic antiserum made against D1.LP antibodies specific to the synthetic terpolymer poly(Glu60Ala30Tyr10), referred to as GAT. This idiotype was found in the anti-GAT antibodies of all individuals of all inbred strains of mice tested. Due to its common (C) occurrence in the anti-GAT antibodies of all mouse strains, it was termed the CGAT idiotype. The presence of CGAT idiotype in mice is independent of the expression of various Ig-1, H-2, or Vg genetic markers. In contrast, antibodies produced in response to the crossreactive poly(Glu60-Ala40), termed GA, bound to the GAT ligand but did not bear the CGAT idiotype. This suggests that GAT, although bearing GA determinants, preferentially stimulates clones of CGAT idiotype. Some of the CGAT idiotypic specificities were also identified in sera of WF inbred rats after immunization with GAT. However, no significant levels of CGAT idiotype were detected in LEW rats, F344 rats, guinea pigs, or rabbits, although measurable quantities of anti-GAT antibody were present.

MeSH Terms
Alanine Animals Cross Reactions Glutamates Guinea Pigs Immunoglobulin Idiotypes Mice Mice, Inbred Strains Peptides/immunology Rabbits Rats Rats, Inbred Strains Species Specificity Tyrosine
Chemicals
Glutamates Immunoglobulin Idiotypes Peptides Tyrosine Alanine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ju S T
Benacerraf B
Dorf M E
References (14)
14 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.12.6192
Published
1978-12-00
Pages
6192-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393145
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.12.6192 PMC Full Text View journal details
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