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PMID: 2827117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of human DNA sequences synthesized at the onset of S-phase.

Nucleic acids research ·Vol. 15 ·No. 24 ·1987-12-23 ·Pages 10211-32

Tribioli C, Biamonti G, Giacca M, Colonna M, Riva S, Falaschi A

Abstract

We developed a method of enrichment for DNA replicated at the onset of S-phase in synchronized human HL60 cells. About 200 such sequences were cloned. The analysis of this selected DNA sample showed that: 1) the cloned DNA fragments derive from a limited number (750-1500) of replicons; 2) there is no extensive homology between different DNA fragments; 3) they are not significantly enriched in highly repeated sequences; 4) they are enriched in snap-back (Cot = o) DNA. The sequence of the longest fragment revealed the presence of numerous signals collected in a few hundred nucleotides: 1) homology with the origin of replication of human Papovaviruses usually associated with potential stem-loop structures; 2) binding sites for known transcription factors and for another nuclear factor; 3) potential binding sites for the chromosome "scaffold".

MeSH Terms
Base Sequence Binding Sites Cloning, Molecular DNA Replication DNA Restriction Enzymes DNA-Binding Proteins/metabolism Humans Interphase Molecular Sequence Data Nucleic Acid Conformation Regulatory Sequences, Nucleic Acid Transcription Factors/metabolism Transcription, Genetic Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins Transcription Factors DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tribioli C
Istituto di Genetica Biochimica ed Evoluzionistica del CNR, Pavia, Italy.
Biamonti G
Giacca M
Colonna M
Riva S
Falaschi A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.24.10211
Published
1987-12-23
Pages
10211-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339940
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.24.10211 PMC Full Text View journal details
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