Defining the beginning and end of KpnI family segments.

The EMBO journal ·Vol. 3 ·No. 8 ·1984-08-00 ·Pages 1753-9

Grimaldi G, Skowronski J, Singer MF

Abstract

Comparison of the sequences at the ends of several newly cloned and full length members of the monkey KpnI family with one another and with previously described monkey and human segments defines the nucleotide sequence at the two termini. No terminal repeats either direct or inverted are noted within full length family members which may or may not be immediately flanked by direct repeats. At the 3' terminus, several family members have polyadenylation signals followed by a d(A)-rich stretch. The genomic frequency of segments within the full length element increases markedly from the 5' to the 3' terminus, consistent with the cloning of various truncated family members. One such truncated version joined to a low copy number DNA segment is inserted in monkey alpha-satellite where the combination appears to have been amplified in conjunction with the satellite itself.

MeSH Terms

Animals Bacteriophage lambda/genetics Cercopithecus/genetics Chlorocebus aethiops/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/analysis DNA, Satellite/genetics Humans Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Species Specificity

Chemicals

DNA, Recombinant DNA, Satellite DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Grimaldi G

Skowronski J

Singer M F

References (27)

27 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1984-08-00

Pages

1753-9

Language

English

Region

England

NLM ID

8208664

PMCID

PMC557592

Subset

Databases

GENBANK

X01019, X01020, X01021, X01022

External Links

PubMed source PMC Full Text View journal details

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Defining the beginning and end of KpnI family segments.

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