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PMID: 2827570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bacillus subtilis generates a major specific deletion in pAM beta 1.

Applied and environmental microbiology ·Vol. 53 ·No. 10 ·1987-10-00 ·Pages 2458-63

van der Lelie D, Venema G

Abstract

pAM beta 1, a 26.5-kilobase plasmid originally isolated from Streptococcus faecalis, was conjugally transferred from Streptococcus lactis to Bacillus subtilis. No conjugal transfer of pAM beta 1 from B. subtilis to S. lactis was observed. In addition, pAM beta 1 which had been reintroduced in S. lactis after cycling through B. subtilis had lost its conjugal transferability to Streptococcus cremoris, although under the same conditions noncycled pAM beta 1 was transferred at high efficiency. Restriction and Southern blot analyses showed that pAM beta 1 had suffered one major, specific 10.6-kilobase deletion and several minor but also specific deletions in B. subtilis. Comparing the major deletion derivative, delta pAM beta 1, with B. subtilis strains which have been reported to contain pAM beta 1 showed that these strains also contained delta pAM beta 1. Hybridization experiments showed that the deleted fragment was not transposed to the B. subtilis chromosome. Based on the size of the minor deletion derivatives from pAM beta 1, it is suggested that these use a different origin of replication in B. subtilis.

MeSH Terms
Bacillus subtilis/genetics Chromosome Deletion Conjugation, Genetic DNA Restriction Enzymes DNA, Bacterial/genetics Lactococcus lactis/genetics Nucleic Acid Hybridization R Factors Streptococcus/genetics Transformation, Bacterial
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van der Lelie D
Department of Genetics, University of Groningen, Haren, The Netherlands.
Venema G
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.53.10.2458-2463.1987
Published
1987-10-00
Pages
2458-63
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC204129
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.53.10.2458-2463.1987 PMC Full Text View journal details
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